FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4092
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA210.5131964809384164No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA180.43988269794721413No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG160.39100684261974583No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC150.36656891495601174No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG150.36656891495601174No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC130.3176930596285435No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT130.3176930596285435No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT120.2932551319648094No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT120.2932551319648094No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG120.2932551319648094No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT110.2688172043010753No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG110.2688172043010753No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG110.2688172043010753No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT110.2688172043010753No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC100.24437927663734116No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC100.24437927663734116No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA100.24437927663734116No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT100.24437927663734116No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT90.21994134897360706No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT90.21994134897360706No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG90.21994134897360706No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG90.21994134897360706No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA90.21994134897360706No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG80.19550342130987292No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG80.19550342130987292No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT80.19550342130987292No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA80.19550342130987292No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA80.19550342130987292No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT80.19550342130987292No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG80.19550342130987292No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG80.19550342130987292No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA70.1710654936461388No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC70.1710654936461388No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA70.1710654936461388No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA70.1710654936461388No Hit
ACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTAT70.1710654936461388No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT70.1710654936461388No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA70.1710654936461388No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT70.1710654936461388No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT70.1710654936461388No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC70.1710654936461388No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA70.1710654936461388No Hit
CCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTGGG70.1710654936461388No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT70.1710654936461388No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT70.1710654936461388No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT60.1466275659824047No Hit
GAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGGA60.1466275659824047No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT60.1466275659824047No Hit
GCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAA60.1466275659824047No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT60.1466275659824047No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA60.1466275659824047No Hit
GAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTG60.1466275659824047No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA60.1466275659824047No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC60.1466275659824047No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG60.1466275659824047No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG60.1466275659824047No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA60.1466275659824047No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC60.1466275659824047No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG60.1466275659824047No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT60.1466275659824047No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA60.1466275659824047No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG60.1466275659824047No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC60.1466275659824047No Hit
ACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAA60.1466275659824047No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG50.12218963831867058No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA50.12218963831867058No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG50.12218963831867058No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA50.12218963831867058No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA50.12218963831867058No Hit
ATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGA50.12218963831867058No Hit
GAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTC50.12218963831867058No Hit
TACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATC50.12218963831867058No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC50.12218963831867058No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA50.12218963831867058No Hit
TCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCT50.12218963831867058No Hit
TTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTG50.12218963831867058No Hit
GACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACAC50.12218963831867058No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA50.12218963831867058No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT50.12218963831867058No Hit
GTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGAT50.12218963831867058No Hit
ACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGA50.12218963831867058No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG50.12218963831867058No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT50.12218963831867058No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC50.12218963831867058No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA50.12218963831867058No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA50.12218963831867058No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA50.12218963831867058No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT50.12218963831867058No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC50.12218963831867058No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG50.12218963831867058No Hit
GTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATC50.12218963831867058No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT50.12218963831867058No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG50.12218963831867058No Hit
GATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGA50.12218963831867058No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA50.12218963831867058No Hit
TCCCTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGG50.12218963831867058No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.12218963831867058No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG50.12218963831867058No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA50.12218963831867058No Hit
CTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGGGG50.12218963831867058No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA50.12218963831867058No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers