FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT15.555555555555555No Hit
ATATTTTCACCGAGTGCCCACTTCAATTGGCTTGTTCTCTTCATGTTCTT15.555555555555555No Hit
GGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGT15.555555555555555No Hit
TACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGGGTTTCA15.555555555555555No Hit
AGAGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGC15.555555555555555No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTTATGAACAATTGAAG15.555555555555555No Hit
GTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGA15.555555555555555No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA15.555555555555555No Hit
CTGTTACTACAATAATCATGTCATCTGATTAATTTACTCCAGACACTAAA15.555555555555555No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT15.555555555555555No Hit
GGATGATGGCAATGAGCTACCCAATTACAGCAGACAAGAGAATAATGGAC15.555555555555555No Hit
CCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATT15.555555555555555No Hit
TTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAG15.555555555555555No Hit
AAACAAGGGTGTTTTTTATAATTAAATAAGCTGAAACTAGAAAGCTCTTA15.555555555555555No Hit
GTTAAATTAATTATTTTTGCCGTCTGAGTTCTTCAAGGGTGGAACAGATA15.555555555555555No Hit
CTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAG15.555555555555555No Hit
GTTTAAGGTCTCCAACATCTTTGCAGACATCAAAGTCTACTATTTCTGGT15.555555555555555No Hit
ACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAG15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)