FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATTCAACACTGTTTAATATAGCTTCCTCTGACTATATTGGGAAGTCAT14.761904761904762No Hit
GAACATATGTATAATCAAGGAGGGGAAGTGAGGAATTATTATTTTTATCA14.761904761904762No Hit
ATATAAATAAGACAGGGACATTTGAATTCTCAAGGTTTTTTTATCGCTAT14.761904761904762No Hit
GGATGATAACAGCAGTAAGCTGTGATAGGAATGTAGGCAATAGTGCGCAT14.761904761904762No Hit
GCCCGGATGATGGCACTGTCCCTGCAGCCCCAGATGCAGGAACATCTGGA14.761904761904762No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGA14.761904761904762No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTAACAACACCACGTCACCTTTC14.761904761904762No Hit
TAGGAACATAGTGAGAAGAGCTGCAGTATCAGCAGATCCACTAGCATCTT14.761904761904762No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGAG14.761904761904762No Hit
CACTAAGATATTCAACATCAAAACTAGCATGTTGCAACGGCATCAAATAC14.761904761904762No Hit
CCAATACATTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGTGATCTGC14.761904761904762No Hit
GATAAGAAGAGTTCACAATGGTTGGGAGAAGCGCAACAGCCAACGTCAGA14.761904761904762No Hit
ATTACATACTGTGCCTCCTTGGCACTGAGATCTGCATGACAAGAAATTAT14.761904761904762No Hit
CACTAAGAGCGGAAAATCCACGCATTCTACTAGAATCAGGCACTCGAGAA14.761904761904762No Hit
CACAAAAACTTACTCATACACAATCATGTAGGAGATTAATGCTGTCTCTT14.761904761904762No Hit
GAATCAAGGCCTCAGTAGATGGGCTGATTTGCCCAAGTGGTGATCGCCCA14.761904761904762No Hit
TGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTGGT14.761904761904762No Hit
ATGGAATACTGGCTAATGCGATCATTCCAGTATCCGAAACACGGATACTA14.761904761904762No Hit
AGTCAGAGGTGATCTGAATTTAGTCAATAGCGCGAATCAGCTATTGAATC14.761904761904762No Hit
GCCCAGGAGGTAATCATGGAAGTTGATTACACTAACGAAGTGGGAGCCAG14.761904761904762No Hit
GTTCGACACAAACACGCACTATAATCTGAATAAATCTCATGTTTGCTTTT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)