FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S4_R1_A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S4_R1_A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG22.631578947368421No Hit
GAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAATGCTAGCAA11.3157894736842104No Hit
TGGATGCTCTGAAATAAAGTATTGAAGCCAAGAGTGACGATGGGGAGGGA11.3157894736842104No Hit
GCCGACACCCTTGACGAAGAGAGCAGGGCAAGCATCAACACTAGGCTTTT11.3157894736842104No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC11.3157894736842104No Hit
GAGTGTACATAAATACGGCCTTGCTCAATGCATCATGTGCCGCCATGGAT11.3157894736842104No Hit
ACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACAC11.3157894736842104No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGCCTCAGTGATGC11.3157894736842104No Hit
GTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAA11.3157894736842104No Hit
TACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGAT11.3157894736842104No Hit
GTCCTATATAAATAAGACCGGGACATTTGAATTCACACGCTTATTTTATC11.3157894736842104No Hit
ATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTC11.3157894736842104No Hit
TTTTATCGCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTT11.3157894736842104No Hit
GCAATATCCTCAAAGGAAAATTTCACACAGCTCCCCAGAGGGCAATGATG11.3157894736842104No Hit
GACCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGCACTCATCCTT11.3157894736842104No Hit
GTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTTCA11.3157894736842104No Hit
GGGCACAGTATATGTAACACAACAGGGGTAGATCAGCCTAAAGTTAGTAA11.3157894736842104No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11.3157894736842104No Hit
CTAACTTGCAGGTCCTGTAGCATCTGTCCACTCCTGCTTGTATTCCCTCA11.3157894736842104No Hit
CCTTACTCCTCCAATCTGTGTGCTGTGGCACCTTTCCAAGAGCGATGCTA11.3157894736842104No Hit
CCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAA11.3157894736842104No Hit
GCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATC11.3157894736842104No Hit
GGATGCCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCA11.3157894736842104No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA11.3157894736842104No Hit
GAATGTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCT11.3157894736842104No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA11.3157894736842104No Hit
CACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGA11.3157894736842104No Hit
TTCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCACTCGGTCAGA11.3157894736842104No Hit
GGTTTTGTCAACTTCTCAGTTCCTTGCGTTTCACTGAATTCTTCGGGAGA11.3157894736842104No Hit
CTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAA11.3157894736842104No Hit
CGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGA11.3157894736842104No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGATAA11.3157894736842104No Hit
GGTCGAAAGGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATC11.3157894736842104No Hit
CACGCTTCGCATTTCACCAACTCATGAATTTAATGTGTGCGTCATGCGGG11.3157894736842104No Hit
TTGGAAACATAATCTCAATATGGATTAGCCACTCAATTCAACTTGGGAAT11.3157894736842104No Hit
GGTCGAAAGGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAACTC11.3157894736842104No Hit
GCCGTAGACTTATGCAAGGCAGCAAAATGGTTGAGGATAAGCTCATCTTT11.3157894736842104No Hit
GCTCAACCCGATTGATGGACCACAACCTGAGGATAATGAACCAAGTGGGT11.3157894736842104No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT11.3157894736842104No Hit
CACCAAGACAATATACAGGTGGGATGGGCTCCAAGCATCCGAAGATTTTG11.3157894736842104No Hit
TTATAAAAGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTT11.3157894736842104No Hit
GTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTC11.3157894736842104No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11.3157894736842104No Hit
GACTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGAC11.3157894736842104No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC11.3157894736842104No Hit
GTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAAACCC11.3157894736842104No Hit
GCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGAAAAAC11.3157894736842104No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT11.3157894736842104No Hit
AGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACA11.3157894736842104No Hit
TAGGATGAGTCCCAATAGTTATCATTTCATGTACAATATGCATAGTCTGA11.3157894736842104No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATAATTGATGA11.3157894736842104No Hit
CCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAACAA11.3157894736842104No Hit
ATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGAT11.3157894736842104No Hit
GACATGTTTACGATATGTTTTCAAAGAATTAATACGTCAAGTCTTTCTCT11.3157894736842104No Hit
AGCAGGTAATGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.3157894736842104No Hit
ATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGC11.3157894736842104No Hit
CCTGAAGTCTGCTTAATATGGGAGCTAATGGATGATGATTATCGGGGAAG11.3157894736842104No Hit
GAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG11.3157894736842104No Hit
AGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTAT11.3157894736842104No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA11.3157894736842104No Hit
CCCTAGAACCACGGCATGTTCCTACTTAGACTTTTCGACCCATTTTGTTG11.3157894736842104No Hit
GTGGCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGAGGCCTTAC11.3157894736842104No Hit
CGGTCGATTCGATCCATGCCAGTTATCCCTGCACACACATGTGATTTCAC11.3157894736842104No Hit
ACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACT11.3157894736842104No Hit
GGATTAAAAAGTCTTCACCGATAATCATCATCCATTAGCTCCCAGTTTAA11.3157894736842104No Hit
AATCCAAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGA11.3157894736842104No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT11.3157894736842104No Hit
ATATGGAGAGAATAAAAGAACAGAGAGATCTAATGTCGCAGTCCCGCACT11.3157894736842104No Hit
GACAAGACCAATCTTGTCACCTATGACTAAGGGAAATTTAGGATTTTTGT11.3157894736842104No Hit
GAGCTCTTCTATCTTATCTTTTGTTATATACAAATATGAATCTGATCACA11.3157894736842104No Hit
CTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCA11.3157894736842104No Hit
ACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCG11.3157894736842104No Hit
GTGCTAACGGGCCACCTACACACCCTTCAAAGAATCGTACAGTAAGGGGA11.3157894736842104No Hit
GGCGATAGCAGGGTGCCAAAAACAGAATGGACTCCACCCCCATGTCAAGC11.3157894736842104No Hit
ATTGGGATCAGCTGAAAGTCATCCATGGCAGCACAGGATGCATTGAGCAA11.3157894736842104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAA50.0145.09
CCATGGA50.0145.01
GGACACA50.0145.05
ATGGACA50.0145.03
ACACAGT50.0145.07
GACACAG50.0145.06
TGGACAC50.0145.04
CATGGAC50.0145.02
CACAGTA50.0145.08
GTGTGCT50.0145.0145
Produced by FastQC (version 0.11.5)