FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATGAAGAATTCCCAATGGATGGGAGCAGAGCAACAGCTATTAGCAGA14.0No Hit
CTTCTTTCTTCTCTTTTGTTATTGCCAGCTGTGACTCTGATGTCAGTATT14.0No Hit
CCGGATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAAT14.0No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC14.0No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA14.0No Hit
ACACAAGGTTCTGGTGATGAGAGGTGGGGTAGCATTGTGGGCTCGCCCAG14.0No Hit
GTGAAAGCCCTTAGAAGTATCAAGGTCTCTAATAGGTTAAAGATTACACT14.0No Hit
AACCTGTATGGGTTCATTATAAAAGGAACGTCTAATTTGAGAAATGATAC14.0No Hit
CCATGGCCTCCACCAGGCTAGAAATTCCACCCGGTCTCCTATATTAACTG14.0No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT14.0No Hit
ATTCTCTTTCTATCATGTACGCCACCATCAAGGGAGCAATGTTAAAAACC14.0No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA14.0No Hit
CAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATGTTG14.0No Hit
TGTACGAATACCTATTTGAGCCCCTGCTTTTAATTCCTTTGGAAATAACA14.0No Hit
GGGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGACCTATTTTACA14.0No Hit
GTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTC14.0No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG14.0No Hit
GGGCAAGTGTGCAGGACCTGACTGGCAAAATCTGTAGTCAACAGTCGATA14.0No Hit
CTTCACATAAAAGCTTCCTAGCATATATTCAGATATTTGTCATAGAAACA14.0No Hit
TCCCCCCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTCT14.0No Hit
CTAAAGACCACTGCTGTTTTAAGTACGTATGCTTGTATTTTTTGTATGCT14.0No Hit
GTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTG14.0No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA14.0No Hit
GCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATC14.0No Hit
TCTCTGTATATTTACAGAGACTCGAACCGTGTTACCATTACATTCACTTC14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)