FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG90.2541654899745835No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT90.2541654899745835No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA90.2541654899745835No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA80.2259248799774075No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT80.2259248799774075No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC80.2259248799774075No Hit
GGGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTT70.1976842699802316No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT70.1976842699802316No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT70.1976842699802316No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG70.1976842699802316No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG70.1976842699802316No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC70.1976842699802316No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA70.1976842699802316No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG70.1976842699802316No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC70.1976842699802316No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC70.1976842699802316No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC60.16944365998305563No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC60.16944365998305563No Hit
CCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCT60.16944365998305563No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA60.16944365998305563No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG60.16944365998305563No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.16944365998305563No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC60.16944365998305563No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG60.16944365998305563No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA60.16944365998305563No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT60.16944365998305563No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA50.1412030499858797No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT50.1412030499858797No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC50.1412030499858797No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA50.1412030499858797No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC50.1412030499858797No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA50.1412030499858797No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG50.1412030499858797No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT50.1412030499858797No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA50.1412030499858797No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA50.1412030499858797No Hit
GCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACT50.1412030499858797No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.1412030499858797No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA50.1412030499858797No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA50.1412030499858797No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC50.1412030499858797No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA50.1412030499858797No Hit
GAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGG50.1412030499858797No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG50.1412030499858797No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT50.1412030499858797No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG50.1412030499858797No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.11296243998870376No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT40.11296243998870376No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG40.11296243998870376No Hit
ACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGT40.11296243998870376No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA40.11296243998870376No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT40.11296243998870376No Hit
TTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCATCCCACCAGTA40.11296243998870376No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT40.11296243998870376No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT40.11296243998870376No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA40.11296243998870376No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT40.11296243998870376No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC40.11296243998870376No Hit
GTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCA40.11296243998870376No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.11296243998870376No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.11296243998870376No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC40.11296243998870376No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA40.11296243998870376No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT40.11296243998870376No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG40.11296243998870376No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC40.11296243998870376No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA40.11296243998870376No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG40.11296243998870376No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.11296243998870376No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT40.11296243998870376No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA40.11296243998870376No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA40.11296243998870376No Hit
GGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAA40.11296243998870376No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.11296243998870376No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.11296243998870376No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.11296243998870376No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG40.11296243998870376No Hit
CCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCCATA40.11296243998870376No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC40.11296243998870376No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG40.11296243998870376No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT40.11296243998870376No Hit
TTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACT40.11296243998870376No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT40.11296243998870376No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA40.11296243998870376No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA40.11296243998870376No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT40.11296243998870376No Hit
GCATTGGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAA40.11296243998870376No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT40.11296243998870376No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA40.11296243998870376No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG40.11296243998870376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAAT100.0067938752145.06
Produced by FastQC (version 0.11.5)