FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158568
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG17580.15173904337078187No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA16940.14621498263373406No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16900.14586972883766858No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC16630.14353926571422654No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT16160.13948253361045707No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT15870.13697944358898226No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA13280.11462426029374194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3400.053.3165661
ATGCGCG450.00964056148.3320127
TATACAG12800.037.3817865
CGAAAGC4950.036.616742
CAGCTCG12500.036.5389989
GCGAAAG5200.034.8608321
ATAGACC8050.030.6216033
ATACAGC17900.028.3511776
GTATTAC13050.028.337451
TACAGCT16000.028.0929817
TATACTG6250.027.8392375
TATATAC26500.027.6325113
TATAGAC17200.027.3986872
GTATATA24150.024.9208031
TACATCA14900.024.8147565
TTACATC14650.024.7444174
TATTACA17250.024.3771952
ATTACAT16450.023.7997683
TAGACCT9450.023.7834434
ACAGCTC19600.023.6728238
Produced by FastQC (version 0.11.5)