FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.19801980198019803No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.19801980198019803No Hit
TAATAATAGGGCTGTTATGAAGATTAGGGAAAAGATTAGGGGAATAAGTA20.132013201320132No Hit
GTTTAAGGATTGTGATAAAGGCCCCACATTTCTTCCTTTTTAATCATAAC20.132013201320132No Hit
GAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCG20.132013201320132No Hit
CACCTACAGTGTTGAGCTGAGGGCTGGTTGTTCCTTTGAAAAATAACAAG20.132013201320132No Hit
GGAGAAAAGGTCACCGAGAAAGTTGTGAAACCTGAAAAGACTGAAAAGAA20.132013201320132No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.132013201320132No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.132013201320132No Hit
GTAAAGTATAACTGACTTTGTGATTGGGAAGTGGGTGCCAAGGAGACCCA20.132013201320132No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA20.132013201320132No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA20.132013201320132No Hit
AACAGATTCTGTGCTGAGCCTGAAGTAGGGCTCTGTCTCAGAACCCTGAG20.132013201320132No Hit
GCCTTCTTCAGTCTCTTCAGCAGATTGAGAGCATGATTGAGGCCGAGTCT20.132013201320132No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.132013201320132No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT20.132013201320132No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)