FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG70.3867403314917127No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC60.3314917127071823No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.22099447513812157No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT40.22099447513812157No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.22099447513812157No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT40.22099447513812157No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC30.16574585635359115No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG30.16574585635359115No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC30.16574585635359115No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.16574585635359115No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA30.16574585635359115No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.16574585635359115No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.16574585635359115No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC30.16574585635359115No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.16574585635359115No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA30.16574585635359115No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.16574585635359115No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA30.16574585635359115No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.16574585635359115No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.16574585635359115No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG30.16574585635359115No Hit
CAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAA20.11049723756906078No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT20.11049723756906078No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG20.11049723756906078No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA20.11049723756906078No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA20.11049723756906078No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA20.11049723756906078No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT20.11049723756906078No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT20.11049723756906078No Hit
TGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGC20.11049723756906078No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT20.11049723756906078No Hit
TCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCG20.11049723756906078No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.11049723756906078No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG20.11049723756906078No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC20.11049723756906078No Hit
GGCACTCCTTCCGTAGAGGGCCCTCTTTTCAAACCGTATTTAAAGCGACG20.11049723756906078No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGC20.11049723756906078No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC20.11049723756906078No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT20.11049723756906078No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA20.11049723756906078No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.11049723756906078No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC20.11049723756906078No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG20.11049723756906078No Hit
GAATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCT20.11049723756906078No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT20.11049723756906078No Hit
AGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGG20.11049723756906078No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG20.11049723756906078No Hit
CTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGG20.11049723756906078No Hit
ATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGA20.11049723756906078No Hit
GTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCT20.11049723756906078No Hit
CTGGGCAGCTGTTTGAAATTTTCCTTTGAGGATATTGCACATTCTTTCAT20.11049723756906078No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC20.11049723756906078No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT20.11049723756906078No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT20.11049723756906078No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA20.11049723756906078No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT20.11049723756906078No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT20.11049723756906078No Hit
GTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTTCCTGGG20.11049723756906078No Hit
GTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCATCAATG20.11049723756906078No Hit
GCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGC20.11049723756906078No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT20.11049723756906078No Hit
CCTCATTTTCCTGGCACGGTCAGCACTCATTCTGAGGGGATCAGTTGCAC20.11049723756906078No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.11049723756906078No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA20.11049723756906078No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC20.11049723756906078No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG20.11049723756906078No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG20.11049723756906078No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.11049723756906078No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAG20.11049723756906078No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.11049723756906078No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA20.11049723756906078No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA20.11049723756906078No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC20.11049723756906078No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA20.11049723756906078No Hit
CTTAAAAATCGGTCAATACTCACTACCACTCTCTCCGTGCTGGAGTATTC20.11049723756906078No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA20.11049723756906078No Hit
CTTCTATGGTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAAT20.11049723756906078No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.11049723756906078No Hit
CCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTG20.11049723756906078No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC20.11049723756906078No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC20.11049723756906078No Hit
CTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACACCAATTGA20.11049723756906078No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20.11049723756906078No Hit
TCCCACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTAT20.11049723756906078No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC20.11049723756906078No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA20.11049723756906078No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA20.11049723756906078No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA20.11049723756906078No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC20.11049723756906078No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC20.11049723756906078No Hit
CAATAAGGCATGTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAA20.11049723756906078No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA20.11049723756906078No Hit
ACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAA20.11049723756906078No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCA20.11049723756906078No Hit
TTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGG20.11049723756906078No Hit
ACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACG20.11049723756906078No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT20.11049723756906078No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA20.11049723756906078No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)