FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCC21.4492753623188406No Hit
TTCCTCCACTCCCCTGGGTGCCTCTCCGATTCGCCCTGAGTCCGATTTGT10.7246376811594203No Hit
GACTCGAACCGTGTTACCATTCCATTCCAGTCCTCCGAGGAGGACCCCAA10.7246376811594203No Hit
AGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTG10.7246376811594203No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT10.7246376811594203No Hit
ATGTGTAGTTGCAACGGCTTCATATTCCATGCTTTTGGCTGGACCATGGG10.7246376811594203No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTATCAG10.7246376811594203No Hit
TAACGGGAAACTATGCAAACTAAGAGGGGTAGCCCCATTGCATTTGGGTA10.7246376811594203No Hit
GGACAAAGAACATCAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAA10.7246376811594203No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC10.7246376811594203No Hit
TACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGAT10.7246376811594203No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA10.7246376811594203No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.7246376811594203No Hit
CTCTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAAT10.7246376811594203No Hit
CCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCT10.7246376811594203No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA10.7246376811594203No Hit
GAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGC10.7246376811594203No Hit
GTTCAGAAACATCCTGAGCATGGCACCCATAATGTTCTCAAACAAAATGG10.7246376811594203No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCCC10.7246376811594203No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT10.7246376811594203No Hit
CCCTGATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGCTACC10.7246376811594203No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA10.7246376811594203No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC10.7246376811594203No Hit
CTGCTGCATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAA10.7246376811594203No Hit
AAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTG10.7246376811594203No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG10.7246376811594203No Hit
GATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCAC10.7246376811594203No Hit
GGTTCCATTGGTTCTCACATATAGGAACATGGGCCTCGACACTTGGCCTA10.7246376811594203No Hit
GGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGA10.7246376811594203No Hit
GATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAA10.7246376811594203No Hit
CTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGACCC10.7246376811594203No Hit
GTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAA10.7246376811594203No Hit
AAAACAAGGTTTCTCCCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGA10.7246376811594203No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC10.7246376811594203No Hit
GATCAAACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAA10.7246376811594203No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC10.7246376811594203No Hit
GTCGGAAGAATGATTGGTGGAATCGGGAGATTCTACATCCAAATGTGCAC10.7246376811594203No Hit
GATGTTATCATCTATGAGAACTCATGATTCATGATCCGTCATCACTAGAA10.7246376811594203No Hit
CCCCTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCA10.7246376811594203No Hit
CCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGT10.7246376811594203No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACT10.7246376811594203No Hit
CTTTGCTGCTGCCCCCCCAGAACAGAGTAGGATGCAATTTTCCTCATTGA10.7246376811594203No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA10.7246376811594203No Hit
GGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAG10.7246376811594203No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG10.7246376811594203No Hit
CTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAGG10.7246376811594203No Hit
CTCCATACTTACAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACC10.7246376811594203No Hit
GCACTGAACTCAAACTCAGTGATTATGATGGACGACTAATCCAGAATAGC10.7246376811594203No Hit
AGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGTTCATT10.7246376811594203No Hit
CTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAATGTTATACA10.7246376811594203No Hit
GAGGCCTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGAA10.7246376811594203No Hit
GCCCTGGTTTTGTGAAGCCCAAAGTTTACACTTCCAAGACCGGCCTCGCC10.7246376811594203No Hit
GTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGA10.7246376811594203No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA10.7246376811594203No Hit
TCTTTTCATTGCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAA10.7246376811594203No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA10.7246376811594203No Hit
ATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCAGGCA10.7246376811594203No Hit
CATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATTTCGG10.7246376811594203No Hit
GCGGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT10.7246376811594203No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG10.7246376811594203No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC10.7246376811594203No Hit
TTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACT10.7246376811594203No Hit
AGATCAGAAAATCGAAACTAACAAGTTTCCTGCAATATGCACACAAAATC10.7246376811594203No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG10.7246376811594203No Hit
GTCTATATGCAGGGAAATTACCCTATAGACGTGATGCTGAAATGAGACGA10.7246376811594203No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA10.7246376811594203No Hit
TATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGC10.7246376811594203No Hit
CTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGT10.7246376811594203No Hit
AGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAA10.7246376811594203No Hit
CAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAG10.7246376811594203No Hit
GGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAG10.7246376811594203No Hit
ACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCATCCACA10.7246376811594203No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG10.7246376811594203No Hit
CTTGACATCGTAACAATATAGTTCTACAGATAGCGGAGGTTTGATTCTGG10.7246376811594203No Hit
GTCCCAAGCCCATCCCGCATTTGCTGGAACAGTGTCCTTACGATTCCCCT10.7246376811594203No Hit
CCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTG10.7246376811594203No Hit
TCACATGTGTGTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTG10.7246376811594203No Hit
GGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAG10.7246376811594203No Hit
GCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGG10.7246376811594203No Hit
GTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCA10.7246376811594203No Hit
CCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAG10.7246376811594203No Hit
CCCTCAGATTACCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTG10.7246376811594203No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAAATTTAGGA10.7246376811594203No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG10.7246376811594203No Hit
GGGTTTTCATTTGGTCTCATCAGGCTGACCACTTGGCTGTTTTGGAGTAA10.7246376811594203No Hit
CAAAAGTGCTTTTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATG10.7246376811594203No Hit
GGTGGTGACAACGGAGATATATTACTGGCCCCCCCTAACACGCAGCCATC10.7246376811594203No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.7246376811594203No Hit
CATGATAAACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAAT10.7246376811594203No Hit
CTCCCCCTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT10.7246376811594203No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT10.7246376811594203No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC10.7246376811594203No Hit
CTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAA10.7246376811594203No Hit
CGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCT10.7246376811594203No Hit
CACAAGAGTCAATTGGTGTGGATGGCATGCCACTCTGCTGCATTTGAAGA10.7246376811594203No Hit
GGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAG10.7246376811594203No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT10.7246376811594203No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.7246376811594203No Hit
GGGACTTTTTGGATTTGTGTTCACGGTCACAGTGGCCAGTGAGCGAGGCC10.7246376811594203No Hit
CACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTC10.7246376811594203No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA10.7246376811594203No Hit
ATTTGAAGATTTAAGAGTATCAAGTTTCATAAGAGGAAAGAAAGTGATTC10.7246376811594203No Hit
GTCATGTCTTTCTCTTTGACAGAAGACTCGGCCTCAATCATGCTCTCAAT10.7246376811594203No Hit
GCCGTGAGGCGGCGAGTGTCGGCCTAGTGGCCGTGTGTCGTGGGATGCGC10.7246376811594203No Hit
AATGTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCG10.7246376811594203No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA10.7246376811594203No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCGTCAATACTTAATTTCAGA10.7246376811594203No Hit
CCATTTTGTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACA10.7246376811594203No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT10.7246376811594203No Hit
CTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCT10.7246376811594203No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC10.7246376811594203No Hit
GGGAGGGAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTT10.7246376811594203No Hit
CACTAGACCAAAAGCAGCATCTGTGGTCACTCAACCCATCCTGTAGTATA10.7246376811594203No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10.7246376811594203No Hit
CCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTC10.7246376811594203No Hit
GACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAG10.7246376811594203No Hit
CAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAA10.7246376811594203No Hit
GTTATACAACAAAATGGAATTTGAACAATTTCAGTCTCTTGTCCCTAAGG10.7246376811594203No Hit
GTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGC10.7246376811594203No Hit
GCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCCCTAA10.7246376811594203No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA10.7246376811594203No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT10.7246376811594203No Hit
ATATAAATAACACAGGGACATTTGAATTCACACGCTTTTTTTATCGCTAT10.7246376811594203No Hit
CTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGC10.7246376811594203No Hit
AATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTA10.7246376811594203No Hit
GATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCA10.7246376811594203No Hit
CCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGA10.7246376811594203No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.7246376811594203No Hit
TTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTC10.7246376811594203No Hit
GCAGGGGAAATCAAAAGCAACAAAAATGAAGGCAAGACTAGTTGATCTCC10.7246376811594203No Hit
CATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATG10.7246376811594203No Hit
AACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAGAAGTGA10.7246376811594203No Hit
GACTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGACCAG10.7246376811594203No Hit
GTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTAT10.7246376811594203No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAA10.7246376811594203No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7246376811594203No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG10.7246376811594203No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers