FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGAAGAAGTCAGTGAAACGCAAGGGACTGAGAAGTTGACCATAGCG12.857142857142857No Hit
CAAAAGGACAAGCGGATCATCAGTCAAGAAAGAAGAAGAAGTGCTAACGG12.857142857142857No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC12.857142857142857No Hit
GCTTAGTGACACCTCCATGGCCCCATGGAACGTTATTTCTCTTTTGAGCT12.857142857142857No Hit
GCTGTGTCAGCAGCCCCATTAGCATCTCTCTTGGAAATGTGCCACAGCGC12.857142857142857No Hit
GTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGC12.857142857142857No Hit
ATTTGACACTGTCCAAATAATAAAACATCTCCCCTTTGCTGCTGCCCCAC12.857142857142857No Hit
GTTTCTCCCAGTAGCCGGCGGACCAGGCAGTGTTTATATTGAAGTGTTGC12.857142857142857No Hit
CTGGAAATCTAGAGGTACCGAGATATGCATTCTCAATGGAAAGAAATGCT12.857142857142857No Hit
GGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGG12.857142857142857No Hit
ATGTTAGACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA12.857142857142857No Hit
CCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTC12.857142857142857No Hit
ATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACAT12.857142857142857No Hit
CCTTCGATCTTGGGGGGCTATATGAAGCAATCGAGGGTTCCAGGTTCCAG12.857142857142857No Hit
CCAGAAATCAACCGGCAGCAACTGCATTGGCCAACACCATAGAAGTCTTG12.857142857142857No Hit
TTTGAAAGTGAACCCACCAAAACTGAAAGATGAGCTAATCCTCAACCCTA12.857142857142857No Hit
CTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATCAAT12.857142857142857No Hit
CATCTTCTACTATTTCATCAAGTACTATCAAGCTAGAATAAATCAATTCA12.857142857142857No Hit
GTGTCGACTGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC12.857142857142857No Hit
GTGGTGAACAGTATATGTACCACAACAGGGGTAGAGAAGCCTAAATTTCT12.857142857142857No Hit
TTTCTAGATTCAGCCGAAAACCCCTCAAGTTGTGGAGACGCATATAGACT12.857142857142857No Hit
GTGTTTAGATTGAAGTGTTTCACTTAACCCAAGGGACGTGCTGGGAGCAG12.857142857142857No Hit
ATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGG12.857142857142857No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG12.857142857142857No Hit
GGTGCTAGCAGAGCTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGA12.857142857142857No Hit
CTATGGATTTGAGGCTAATTTTAGCATGGAGATACCCAGCTTTGGAGTGT12.857142857142857No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT12.857142857142857No Hit
ACCAGAAGCCGGTACAGTGGATTCGTAAGGACACTGTTCCAGCAAATCCG12.857142857142857No Hit
ACAATGCTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAG12.857142857142857No Hit
GAGAACTCTTCTTTCTTGCTCCGTCCAGACTCGAAGTCGACCCTGGCATC12.857142857142857No Hit
GGTGTTCAAGTGTGCTTCTAATCGGACAGATCCACATTTAGGTGTCATAG12.857142857142857No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT12.857142857142857No Hit
TCCTTACTCCTACAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT12.857142857142857No Hit
CACATATAGGAACATGGGCCTCGACACTTGGCCAATCGCAGTCCTCAAGA12.857142857142857No Hit
GACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACATGC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)