FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT30.2958579881656805No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC30.2958579881656805No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC30.2958579881656805No Hit
ATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTA30.2958579881656805No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT30.2958579881656805No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30.2958579881656805No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCT30.2958579881656805No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT30.2958579881656805No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT20.19723865877712032No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG20.19723865877712032No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA20.19723865877712032No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG20.19723865877712032No Hit
AGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCAT20.19723865877712032No Hit
CATTCGAAGCAACTGGAAATCTAGTGGTACCGAGATATGCATTCGCAATG20.19723865877712032No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.19723865877712032No Hit
AGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAAC20.19723865877712032No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT20.19723865877712032No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.19723865877712032No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAG20.19723865877712032No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA20.19723865877712032No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA20.19723865877712032No Hit
TTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTA20.19723865877712032No Hit
CCACTAACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCC20.19723865877712032No Hit
CAAGAATACTGACATCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAA20.19723865877712032No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA20.19723865877712032No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT20.19723865877712032No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA20.19723865877712032No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT20.19723865877712032No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG20.19723865877712032No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA20.19723865877712032No Hit
GTTCCAGGGTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATC20.19723865877712032No Hit
ATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACT20.19723865877712032No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.19723865877712032No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT20.19723865877712032No Hit
ATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATG20.19723865877712032No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA20.19723865877712032No Hit
TTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG20.19723865877712032No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.19723865877712032No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.19723865877712032No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT20.19723865877712032No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT20.19723865877712032No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.19723865877712032No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA20.19723865877712032No Hit
GGTCCAAGGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAAT20.19723865877712032No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC20.19723865877712032No Hit
TCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAAATTAA20.19723865877712032No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT20.19723865877712032No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT20.19723865877712032No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.19723865877712032No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.19723865877712032No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers