FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCTTACCAATTGCCATGGAATCTTATGAGTTAAGTCAGAGGTT14.545454545454546No Hit
GTGTTTTATTACCACGGTTAATTAGGAAACAGTGGTCTACCACTACACAT14.545454545454546No Hit
GGCTGGACCTGTCCGGGTCACCTGGGGAGAGGCAGTAGGCAGGGGGTTTA14.545454545454546No Hit
GTTTCCAAATGGGCTTGCTGTCATTAGGTTAGCTACCCAGTACGTTCCAT14.545454545454546No Hit
GTGAGGAGTAAGAGCCGCTGCCCTGGGTCACTTTTGACACTTGTATTTTA14.545454545454546No Hit
GGCTTGTTGCTCTGTACCATTACGCACCCCAGGTCCTTCTAGAATCACGC14.545454545454546No Hit
CCAGGGGCCGCGGCGAGGGCTGGGCTAAGCGGCCTGGAGGGCCCGAGAGG14.545454545454546No Hit
CTATAGTATAACAATTTCTGGAATCAAGAGTAATATTTTTTAAGGGAAAG14.545454545454546No Hit
CATTTTTATAGCACACTGTTATTTTGTTGGGTTAACTTGACCTGTACTGT14.545454545454546No Hit
GTTAGAAATTTCTATTGACATTTGTTTAGGCTTACTGATTCTCTCCTTCG14.545454545454546No Hit
GGGTCGGGGTGTGTGTTGTGGTCCCGTCACAGACTGTCGTCTGTTTGGAC14.545454545454546No Hit
GTTTTATTATTTGCACATTCTCAAATGTACCTTCCACATACAAAAATTGC14.545454545454546No Hit
ACAAAAATAATGAATACTGTTTATTCTTAAAATAAAAATTAATTTAAAAA14.545454545454546No Hit
GTCCCGGGCTGGAATCCTGGCTCCGGGGATGCACATTGGGTGCAATCATA14.545454545454546No Hit
GTCCAATCCTTAAGGTATGCCCATTGTATACGTGAAAAGCTTTTGAAACA14.545454545454546No Hit
CGGAGGGCGTGCGGGCCTGCAGCAGCGTGGCGGCGGTGCAGGTGTGGGAC14.545454545454546No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGAGGAATACTCCAGCACGGAGAGA14.545454545454546No Hit
CAATTCCTCCCTCCACACAGAAAGCCCAAACTACTAACAACCACTCAAAT14.545454545454546No Hit
CGAGTAGAGAAAATAATTTTTTGATGGCATTTTTTAACCGTTTCCCAGGG14.545454545454546No Hit
GGAAAAAACATACGCATACACTCAAATTGTCATTGTTGATTAAAGTTTTA14.545454545454546No Hit
GTGTTTATATGGAGTGAATTTAGTAAGAGTAACTTGTTGCAGTGCTGCAT14.545454545454546No Hit
GAGCAGAGATCGTGTCTTCATGCACTTTTCCATCCCCAGGGCCTAGACGA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)