FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG150.4832474226804124No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT110.3543814432989691No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC100.3221649484536082No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC100.3221649484536082No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA90.28994845360824745No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA80.25773195876288657No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.25773195876288657No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC70.22551546391752575No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT70.22551546391752575No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA70.22551546391752575No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT70.22551546391752575No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG70.22551546391752575No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT70.22551546391752575No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA70.22551546391752575No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC60.19329896907216496No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA60.19329896907216496No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.19329896907216496No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG60.19329896907216496No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA60.19329896907216496No Hit
TCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGAG60.19329896907216496No Hit
TACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCC60.19329896907216496No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT60.19329896907216496No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC60.19329896907216496No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC60.19329896907216496No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT60.19329896907216496No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT50.1610824742268041No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG50.1610824742268041No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA50.1610824742268041No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT50.1610824742268041No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA50.1610824742268041No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC50.1610824742268041No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT50.1610824742268041No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.1610824742268041No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA50.1610824742268041No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA50.1610824742268041No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT50.1610824742268041No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG50.1610824742268041No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.1610824742268041No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.1610824742268041No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT50.1610824742268041No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT50.1610824742268041No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.12886597938144329No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG40.12886597938144329No Hit
GAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAATAC40.12886597938144329No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACA40.12886597938144329No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.12886597938144329No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG40.12886597938144329No Hit
AGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTT40.12886597938144329No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA40.12886597938144329No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG40.12886597938144329No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC40.12886597938144329No Hit
GGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC40.12886597938144329No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT40.12886597938144329No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT40.12886597938144329No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.12886597938144329No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT40.12886597938144329No Hit
GTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGC40.12886597938144329No Hit
GTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAA40.12886597938144329No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC40.12886597938144329No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC40.12886597938144329No Hit
CTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTGT40.12886597938144329No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT40.12886597938144329No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA40.12886597938144329No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT40.12886597938144329No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA40.12886597938144329No Hit
GAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACG40.12886597938144329No Hit
GAGCTACCCAGCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAG40.12886597938144329No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAA40.12886597938144329No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG40.12886597938144329No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.12886597938144329No Hit
CCTGTATGGGTTCATTATAAAAGGAAGGTCTCATTTGAGAAATGATACTG40.12886597938144329No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT40.12886597938144329No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC40.12886597938144329No Hit
GAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAA40.12886597938144329No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA40.12886597938144329No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG40.12886597938144329No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA40.12886597938144329No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG40.12886597938144329No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC40.12886597938144329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGG100.0067558195145.03
ATGGGTG100.0067558195145.05
TGGGTGC100.0067558195145.06
GGGTGCC100.0067558195145.07
Produced by FastQC (version 0.11.5)