FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAATTGATCTTCT13.125No Hit
CATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATT13.125No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13.125No Hit
GCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT13.125No Hit
AGGCATTTACAAAAAGATGCAAAAGTGATTTTCCAGAACTTGGTAATTGA13.125No Hit
GACATTCTCAACGCTAAGTACGCTTAACATGCCGTCAGGAGGCGTACCAG13.125No Hit
GGTCAAGATATAAGAACGTGGATTTGGATTTCCCTTGGACTGAATTATCC13.125No Hit
GGGTGAACCCACTGAGCCACTCAGGGTCCACACGAGGTGACTTTCGCCCC13.125No Hit
CATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCGTCAATACTT13.125No Hit
AGAAGATCCCAAGGACAAAGAACATTAAGAGAAAAACCCAATTCAATTGG13.125No Hit
CCTTCAGTGCATGTGTGAGGTAGGAGTTGAACCAAGATGCAGTAAGAAAA13.125No Hit
GATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGACAT13.125No Hit
CTGTAATATTTGTTGAGTCTTTACAACTGACTTGTTTGCCAGATGCTGGA13.125No Hit
CCTCCAGTCCCATCCATGTTGATGCAAAAGTTGTGTATTCATCCTTTCTG13.125No Hit
CCTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAA13.125No Hit
CCGAGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC13.125No Hit
CAATGTTCAGTTTATCCAACTTAGGGTTAGCAAAAATGAGCAATTACAAG13.125No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA13.125No Hit
ATTAGAAGCATCTTTTGATCTCAAATTTGGTCTTTGCCCTTACTTCCTGA13.125No Hit
CCCCTGGGTGACTCTCAGATTGGCCATGTTTCAGATTTGTTTTAAAAGAA13.125No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA13.125No Hit
CTTTTGGTCGCTGTATGGATGTCAGTAAGTATGCTAGTGTCCCGGTTTCG13.125No Hit
TGAGAAAGTTTTTGGAAGGTGCTACCATAGAAGTAGATCAAATTCATACG13.125No Hit
CACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTA13.125No Hit
TTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAATTTTAG13.125No Hit
ACAATAGAGAGGATGGTTCTTTCTGCTTTTGATGAGAGAAGAAAAAAATA13.125No Hit
GCGCCGGCAGGGCGGCTGCGGGACGGGCGAGAACCGGCAAAGCCGGGCGG13.125No Hit
ATTCCAGCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCT13.125No Hit
CAATTGACACTGTCCAAATAATAAACCTTCTCCCCTTTGCTGCTGCCCCA13.125No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC13.125No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG13.125No Hit
GGCCCCGCCCCCGGCCCCCCCCCCGTCCGGGCAGGCCTTTGGGGGGGCTG13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)