FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGGAGGAACTATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTG21.5384615384615385No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG21.5384615384615385No Hit
GTTCTGCACAGACAGCAGAACACCGGAGCTCCGGGTACGTGAGGCATCTG10.7692307692307693No Hit
GCGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCA10.7692307692307693No Hit
GATAAATACATTTGAAAAAAAGACGCTCAGTAATCCACAATATCAGGTGC10.7692307692307693No Hit
CAATTGGCTTGTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCAT10.7692307692307693No Hit
AGAGAAGCCTAAATTTCTTCCTGATTTGTATGATTACAAAGAGAACCGGT10.7692307692307693No Hit
GTCCTGGAGTGAGGAGAGAAGTGATGGTTCTTGAAATACAAACACAGTCA10.7692307692307693No Hit
GACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCC10.7692307692307693No Hit
TGGTAGATGGATGGTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGA10.7692307692307693No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT10.7692307692307693No Hit
ATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTAT10.7692307692307693No Hit
CCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTC10.7692307692307693No Hit
GACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCA10.7692307692307693No Hit
GTTCAGAAACATCCTGAGCATGGCACCCATAATGTTCTCAAACAAAATGG10.7692307692307693No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC10.7692307692307693No Hit
CTCGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGAACTTT10.7692307692307693No Hit
CCATGTAACAGGAACAGGATACACCATGGACACATTAAAAAGAACAACCC10.7692307692307693No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.7692307692307693No Hit
GGGTGTGCTGGTAGGTGCAATTTGCTCTAAGGTATTTTTGAACCAGCTGG10.7692307692307693No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA10.7692307692307693No Hit
ATCCAGAAATGCTTACTACATTATTAAAAACATGGAAGCAAATGAAAGAG10.7692307692307693No Hit
GGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGC10.7692307692307693No Hit
CAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGT10.7692307692307693No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG10.7692307692307693No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG10.7692307692307693No Hit
TTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTG10.7692307692307693No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC10.7692307692307693No Hit
CAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACA10.7692307692307693No Hit
TAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTT10.7692307692307693No Hit
CCCATACACACAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCCTCA10.7692307692307693No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG10.7692307692307693No Hit
GCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAG10.7692307692307693No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT10.7692307692307693No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.7692307692307693No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG10.7692307692307693No Hit
CACTCGATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGC10.7692307692307693No Hit
CCCAGAATGTGCTCTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGG10.7692307692307693No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT10.7692307692307693No Hit
AGTCAATTCACATGCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAG10.7692307692307693No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT10.7692307692307693No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA10.7692307692307693No Hit
GGGCACGGACTGGGCGCACTGAGCCCAGTGCCTTTCGCCTTAGGTTCAGA10.7692307692307693No Hit
AGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACA10.7692307692307693No Hit
AACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGG10.7692307692307693No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCAACATCTTCTCCTATTTCAT10.7692307692307693No Hit
CCGCCACCGCCCGCGGCTGGGCCGGTGGATCGTCTGCCCTCAGGGCCTCC10.7692307692307693No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT10.7692307692307693No Hit
GCTTCAGCTCAGGCAAATGATGAAGGCTAAACAGCTTGTGAAAGACGTGA10.7692307692307693No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC10.7692307692307693No Hit
CTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGA10.7692307692307693No Hit
GCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAGCTTGCGCATAGTTC10.7692307692307693No Hit
CTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCC10.7692307692307693No Hit
ACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAG10.7692307692307693No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA10.7692307692307693No Hit
GTTCAGAAACATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGG10.7692307692307693No Hit
TACTACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTA10.7692307692307693No Hit
GTATAAGGAATACAGTCAGTGATACTGTCGTAGCGTGTGGTGACGCATGG10.7692307692307693No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG10.7692307692307693No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA10.7692307692307693No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT10.7692307692307693No Hit
CTGAACAGCCTATTCCATTAAGTTGCATAAGACGTAAACCTGTTGTGGCG10.7692307692307693No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC10.7692307692307693No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT10.7692307692307693No Hit
GAAGAGAAGATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAA10.7692307692307693No Hit
GATCAACAAAACGTGCCCCATGCATATCAGCCTTAAGCATGGAAATTCTG10.7692307692307693No Hit
AGGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCAC10.7692307692307693No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA10.7692307692307693No Hit
GAGCGAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT10.7692307692307693No Hit
ATCTTGGACAAAAGAAATACACCAAGACAATATACTGGTGGGATGGGCTC10.7692307692307693No Hit
GGCTCTCGACGTGCACCGAGCCAAGACGCTAGCCATATACCTACCGTTCA10.7692307692307693No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC10.7692307692307693No Hit
GGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTGGAAG10.7692307692307693No Hit
GTGTGTATGCCTATCATGGGTTGTTATGTTTTGTCGTGTGTTTGTGGTGT10.7692307692307693No Hit
CACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGA10.7692307692307693No Hit
TCCTTAGACAGAATCCAACTGAGGAACACGCCGTATACATATGCAAGGCA10.7692307692307693No Hit
ATTCCTTTCATTCCAGAGATGCAACAGCCTCTCCTTCCCCCAGCCGTGGT10.7692307692307693No Hit
GCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAA10.7692307692307693No Hit
GCTTTGTCAATGCTTCAATCCAATGATCGTCGCGCTTGCGGGAAAGGCAA10.7692307692307693No Hit
ATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGCTG10.7692307692307693No Hit
TCCCGCATTTGCTGGAACAGTGTCCTTACGAGTCCACTGTACCGGCGTCT10.7692307692307693No Hit
TTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAG10.7692307692307693No Hit
CCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTT10.7692307692307693No Hit
CTTTCAAAGAAAAAGGAGAGTAAGCGACAACATGCCCAAGAAGATGGTCA10.7692307692307693No Hit
GGGCTACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTC10.7692307692307693No Hit
ACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTT10.7692307692307693No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA10.7692307692307693No Hit
GTGAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAAT10.7692307692307693No Hit
TTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGA10.7692307692307693No Hit
TTCTGGTGCCATATTTTCACCGAGTGCCCACTTCAATTGGCTTGTTCTCT10.7692307692307693No Hit
CCATTCTTGAGGACGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCT10.7692307692307693No Hit
ATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGA10.7692307692307693No Hit
ATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACA10.7692307692307693No Hit
ACACAGAAATACATTTAACACATCAAGAATCGTATCTTTAACAATAAAAA10.7692307692307693No Hit
CCAGTAAAATGTACACGTGGCTGGATCCAGCCCCAGAGGTCTCCACTCAC10.7692307692307693No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG10.7692307692307693No Hit
AGTCAAATCAGTCGAAATGAATGCCCCTAATTATCACTATGAGGAATGCT10.7692307692307693No Hit
GTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTAT10.7692307692307693No Hit
CATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCG10.7692307692307693No Hit
GTATTGAAGACCCGAGTCACGAGGGGGAGGGAATACCACTATATGATGCA10.7692307692307693No Hit
GGCGGCAGCTGGGTCTGCGGTGATGGGATGTAGGGCCGGTAGCAGGCTGG10.7692307692307693No Hit
TTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGG10.7692307692307693No Hit
GGAAATAGGTTTAATCACGATAATCAGGAAACGTAAGATAAAAGCACAAC10.7692307692307693No Hit
AACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGC10.7692307692307693No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG10.7692307692307693No Hit
TGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATTGGGCATGGAAA10.7692307692307693No Hit
ATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCG10.7692307692307693No Hit
CATCTGCATTCTCCGTGTCCGTGGCCTCAGTCTCTGGACAGGGTGCTGGC10.7692307692307693No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT10.7692307692307693No Hit
ATGTCTTAGAGAATCCCGGTGGAACCTCCACACCATGTCTTTGGGACCCC10.7692307692307693No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG10.7692307692307693No Hit
CTATGGCACTGAGGAGTACCTGCACCCCGACATGTATGAGCGGGCCGTGC10.7692307692307693No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT10.7692307692307693No Hit
CGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACAGCAGAAGTG10.7692307692307693No Hit
ATATGATGCAATCAAATGCATGAAGAATTTATATGGCTGGAAAGCGCCAA10.7692307692307693No Hit
GCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGAT10.7692307692307693No Hit
GTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTC10.7692307692307693No Hit
GAATGGAATGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGC10.7692307692307693No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC10.7692307692307693No Hit
CTATAAAACAAGTATTCCCTTAACAACCCACTTAACAACTAAAACTGTAT10.7692307692307693No Hit
GTCAATTGCGGAGGCTATAATTGTGGCCATGGTAGTCTCACAGGAGGCTT10.7692307692307693No Hit
GCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATA10.7692307692307693No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC10.7692307692307693No Hit
CGCATAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCT10.7692307692307693No Hit
GGGGAGCAGGTCTAATGAGGGGCCCAAGCCACTTCTCTCTAGCACCCCTT10.7692307692307693No Hit
TACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACATTCTTGCCCC10.7692307692307693No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG10.7692307692307693No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers