FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTTTTGCAAGATTCCTCAGTTCATTGATGCTTAATGATGGTCAATAT14.545454545454546No Hit
ATCCAATGATCGTAGACCTTCCGGAAAACGCAAAGAAAGAATATCCGGAA14.545454545454546No Hit
CCTCTGGCTAAGGGAATTTTAGGAGTTGTGTTCACGCTCACCGTGCCCAC14.545454545454546No Hit
AGTAAGAAGAGCAGCAGTGTCAGCAGACCCAGTAGCAGCTAGCGGGGAAA14.545454545454546No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGCTTCATTTA14.545454545454546No Hit
TCCCAGGACTCAGTGATGCTGTGCAATCTATAAGAAGAGGCCATATTTTC14.545454545454546No Hit
GAACTTATCCTTTATGACAAAGAAGAAATAAGGAGATTTTGGCTAAAAGC14.545454545454546No Hit
TCTCTGATATGTGGCATCATTCAGGTTGGAATGCCAAATCATGATATGAG14.545454545454546No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATT14.545454545454546No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCA14.545454545454546No Hit
AATGAAAACCCAGCTCACAAGAGTCAATTGGTGTGGATGGCATGCAACTC14.545454545454546No Hit
AGTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAG14.545454545454546No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTATCGAAAATTTTCATTTC14.545454545454546No Hit
ATTCCAGTCCTCCGATCAGGACCCCACCAGAATTTTTGACATCCTCATAA14.545454545454546No Hit
TCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATT14.545454545454546No Hit
CCATATGGCCATAATAAAAAACTACACATCAGGAAGGAAAGAGAAGAACC14.545454545454546No Hit
GAGGAAATAGGAGAAGATGGTGCAACGATTTAACATATCTAAAGCATTAG14.545454545454546No Hit
ATTTAACAAAACAGCATTGTTCAGTAAGCACGCCCGCCAGATTTTACAAA14.545454545454546No Hit
TTAGTAGAAACAAGGCATTTTTTCATGAAGCACAAGTTAAATTCATTATT14.545454545454546No Hit
GATTCAGGGGATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCC14.545454545454546No Hit
AGACTGAAATGGTTCAAATTCCATTTTGTTGTATATCATTGTGGGATCTT14.545454545454546No Hit
GAATATGATGCCGTTGCAACAACACATACCTCGATTACCAAGAGGAATCG14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)