FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.3088803088803089No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.3088803088803089No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.3088803088803089No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG30.23166023166023164No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.23166023166023164No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.23166023166023164No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA30.23166023166023164No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC30.23166023166023164No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.23166023166023164No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.23166023166023164No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.23166023166023164No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.23166023166023164No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT30.23166023166023164No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.23166023166023164No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT30.23166023166023164No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC20.15444015444015444No Hit
CTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTT20.15444015444015444No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT20.15444015444015444No Hit
TATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAG20.15444015444015444No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC20.15444015444015444No Hit
CCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTACAGGACATTGAAAATG20.15444015444015444No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG20.15444015444015444No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA20.15444015444015444No Hit
CCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACC20.15444015444015444No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT20.15444015444015444No Hit
GGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCCCATTC20.15444015444015444No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.15444015444015444No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC20.15444015444015444No Hit
GGCTCCAATCATCCGACGATTTTGCTCTCATAGTGAATGCACCAAACCAT20.15444015444015444No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC20.15444015444015444No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG20.15444015444015444No Hit
ATGCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCAT20.15444015444015444No Hit
ATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAG20.15444015444015444No Hit
GCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACT20.15444015444015444No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.15444015444015444No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.15444015444015444No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.15444015444015444No Hit
GAGTAAGAGACAACATGCCCAAGAAGATGGTCACGCAAAGAACAATAGGG20.15444015444015444No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC20.15444015444015444No Hit
AGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTG20.15444015444015444No Hit
GTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGA20.15444015444015444No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA20.15444015444015444No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC20.15444015444015444No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT20.15444015444015444No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT20.15444015444015444No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20.15444015444015444No Hit
ATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAAT20.15444015444015444No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT20.15444015444015444No Hit
TCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCTGTAGAATCTG20.15444015444015444No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC20.15444015444015444No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT20.15444015444015444No Hit
CAATTGAAGAGCCATCTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCA20.15444015444015444No Hit
TCCGTATATTGTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACC20.15444015444015444No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT20.15444015444015444No Hit
GCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTC20.15444015444015444No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA20.15444015444015444No Hit
GCCTAACATAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCA20.15444015444015444No Hit
CCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACAC20.15444015444015444No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG20.15444015444015444No Hit
CATATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGC20.15444015444015444No Hit
GGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAA20.15444015444015444No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA20.15444015444015444No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC20.15444015444015444No Hit
GAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGG20.15444015444015444No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT20.15444015444015444No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG20.15444015444015444No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG20.15444015444015444No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)