FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGTCATTACGGGGTAAGTGACATCGTACGTGGAAGAGTAAGGTACAAA15.555555555555555No Hit
CCACTGGCCTGTATGATTGCGCTTACCTGTTAATTCTTCCTCTAATCCAC15.555555555555555No Hit
CAACAATGCTGTCCCCAGGGGTATGCACATACAGTGCCTTTGTAATCCAC15.555555555555555No Hit
ATGAGATACAGCATCAGGTAAGGACTTATTTATTTAACTGAATTGAGTTC15.555555555555555No Hit
GGGGAAGTGGTAGATGGAAAAGGAGAAGGAAGCTTCCATCTGAGCAGTAA15.555555555555555No Hit
CTTCAAGAACACCTTTAACTTTGCAGAATTTTTTATGTATTTTTTTAATC15.555555555555555No Hit
GTGTATCCTGTCATTTTGGTGTATTATATTTGTATTTTTGTCGGTATCAA15.555555555555555No Hit
GTATATAATGATTTCAATGGGAAAATGAGTTAAATGTTTCTTCCCTATAA15.555555555555555No Hit
GGTCATGTTCCCTCTCTCTCTAAAATAAAAAATCTTAAAAAAAAAAAAAA15.555555555555555No Hit
GTCATTGATTCCACGTTTGATCATTCTGATTAACTCCATTGCTAAAGTTC15.555555555555555No Hit
CTCCCAAAACTTAACAATGCATGTACCATCACCCAAGTCTGATTAGCAAA15.555555555555555No Hit
TCCCGGAAATGTCCTCCTCACCTAGAAGAACCGTCTGTGTGTTGGCTGGT15.555555555555555No Hit
CCTGTAGTCTACTCAGTTGCAAGATAACATCGTCATCCTTCTTGTCACCA15.555555555555555No Hit
GACCACTACCCCTGATGCTGCGGCATGTTCCCTCCTCAGTCTTCTCCCCC15.555555555555555No Hit
GGAATAAAAACAAAACTAAACTAACGTTCTTAGTTTGCTTTTTAACTTCT15.555555555555555No Hit
AAGCAGGAGTGGGTCCTCCCTCCAGATTTACACGGGAAGCTGTAACTTCT15.555555555555555No Hit
GAGGTAACCTTTGAATACAGATCTGAAGACAAGGGAGTGAGCAATGTGAA15.555555555555555No Hit
GTACTTATTAAACGGTGAGAGTACATACACGTTTAGATAATAAAGAAAAA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)