FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAA13.4482758620689653No Hit
ATTAGCACCCTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATCT13.4482758620689653No Hit
TGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATGTCT13.4482758620689653No Hit
GGGCCGAAGGTACCATGTTTCAACCTTTCGCCCTTTTCGAAATAAGTTTT13.4482758620689653No Hit
GTTATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGA13.4482758620689653No Hit
GCTACAGGACAGTGAAAATGAAGAGAAGATCCCAAGGACACAGAACAGGA13.4482758620689653No Hit
GGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGCACACAAAT13.4482758620689653No Hit
GAATACATTATGAAGGGAGTGTACATCAATACGGCCTTGCGCCATGCATC13.4482758620689653No Hit
GTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAAGA13.4482758620689653No Hit
GGTTGTTATCTCTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTA13.4482758620689653No Hit
GCGCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA13.4482758620689653No Hit
ACATTTGCCAGTTTGGCCTTCTTTTCATTGCCCCCTACTGGGAGCCCAGA13.4482758620689653No Hit
ACATGATAAACAATGCCCTTGGACCTGCAACGGCCCAGATGGCTCGTCAA13.4482758620689653No Hit
GCGCTTATCGATTGTTCTACGTCAATCAGTAGTTGTAAGGCTGGCATAAA13.4482758620689653No Hit
GTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGA13.4482758620689653No Hit
CTTCTAATAGATGGCACAGCATCACTGATGCCTGGGATGATGATGGGCAG13.4482758620689653No Hit
GTGGAATGAAATTCAAAATACTCGAATGTTCCTGGCGATGATTAGATAGA13.4482758620689653No Hit
ACACAGTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGA13.4482758620689653No Hit
GTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGAT13.4482758620689653No Hit
GTTATTTAGTCAGTAGAACTTAATGGTGACAATGAGTAGTGAACAGCGTT13.4482758620689653No Hit
GATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACT13.4482758620689653No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG13.4482758620689653No Hit
GGTGTCTGTCTAAGGATGGCCCCCCTCCGTCCTCATCCCATCTGTGTGCT13.4482758620689653No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG13.4482758620689653No Hit
GAGCAAGGCCGTATTTATGTCCACTCCCTTCATTATGTATTCAGTAGCCC13.4482758620689653No Hit
TCCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGC13.4482758620689653No Hit
AATATATTCAATATGGAGAGAATAAAATAACTGAGAGATCTAATGTCGCA13.4482758620689653No Hit
GGTTAAGTGTGAGGAAGCGTATTAGTTTTGTTATTCCTACTTAACCTTTG13.4482758620689653No Hit
ATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGG13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)