FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R2_F08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC14.761904761904762No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC14.761904761904762No Hit
TGATGACACTGAGCTCAATTGCTCTCTTAGCTCCTCATAATCGATGAAAT14.761904761904762No Hit
CAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGG14.761904761904762No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14.761904761904762No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGT14.761904761904762No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC14.761904761904762No Hit
TGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCAT14.761904761904762No Hit
ATATAGCCATAATCAAAAAGAACACATAAGGAAGGCAAGAGAAGAACCCC14.761904761904762No Hit
CCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAA14.761904761904762No Hit
AATGGAGACTGGTGGGGAGCGCCAGGATGCCACAGAAATCAGAGCATCTG14.761904761904762No Hit
AGAATGAAGTGGATGATGGCAATGACATCCACAATTACAGCAGACAAGAG14.761904761904762No Hit
CCATTTGAATGGATGTCAATCCGACTCTAATTTTCCTAAAAATTCAAGAG14.761904761904762No Hit
ACTCCATACTTACAAAGTTCACCACAGCAGTATCATTTCTCAAATGAGAC14.761904761904762No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA14.761904761904762No Hit
TTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTG14.761904761904762No Hit
CTCTTATCCCTCTAAGCGACATCTCCGTGCTTGGTGTCATGTAGGGCAGT14.761904761904762No Hit
CGCATATAGACTGTTGAATACAGATTTTGCAAGTAATTACCTGCACACTT14.761904761904762No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC14.761904761904762No Hit
GAGACTGGAAAGTGGCTTTGCAGGAAAGCACACAGATCTTGAGGCTCTCA14.761904761904762No Hit
GGTTAATGCTGGGCCATATCTCATGTCTTCTTTGCCCAAAATGAGAAATC14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)