FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC130.4813032210292484No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.40725657164013324No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC110.40725657164013324No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT90.33320992225101814No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG90.33320992225101814No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT90.33320992225101814No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG90.33320992225101814No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG90.33320992225101814No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA80.2961865975564606No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA80.2961865975564606No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80.2961865975564606No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT80.2961865975564606No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG70.259163272861903No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG60.22213994816734545No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50.18511662347278787No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG50.18511662347278787No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG50.18511662347278787No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG50.18511662347278787No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.18511662347278787No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT50.18511662347278787No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA50.18511662347278787No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACTGGATTCCCAAG40.1480932987782303No Hit
AGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGG40.1480932987782303No Hit
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC40.1480932987782303No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.1480932987782303No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC40.1480932987782303No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG40.1480932987782303No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG40.1480932987782303No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40.1480932987782303No Hit
CCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCTTA40.1480932987782303No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG40.1480932987782303No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC40.1480932987782303No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT40.1480932987782303No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA40.1480932987782303No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC40.1480932987782303No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.1480932987782303No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.1480932987782303No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTG30.11106997408367272No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC30.11106997408367272No Hit
CAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCT30.11106997408367272No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA30.11106997408367272No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA30.11106997408367272No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG30.11106997408367272No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA30.11106997408367272No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA30.11106997408367272No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT30.11106997408367272No Hit
ATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAA30.11106997408367272No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.11106997408367272No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG30.11106997408367272No Hit
GTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGGGGTGAATC30.11106997408367272No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT30.11106997408367272No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA30.11106997408367272No Hit
GCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTC30.11106997408367272No Hit
CTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGAC30.11106997408367272No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC30.11106997408367272No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG30.11106997408367272No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG30.11106997408367272No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA30.11106997408367272No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT30.11106997408367272No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA30.11106997408367272No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.11106997408367272No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC30.11106997408367272No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30.11106997408367272No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT30.11106997408367272No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG30.11106997408367272No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.11106997408367272No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG30.11106997408367272No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG30.11106997408367272No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC30.11106997408367272No Hit
ATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCC30.11106997408367272No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA30.11106997408367272No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.11106997408367272No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG30.11106997408367272No Hit
GATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACA30.11106997408367272No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC30.11106997408367272No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.11106997408367272No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT30.11106997408367272No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT30.11106997408367272No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA30.11106997408367272No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA30.11106997408367272No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA30.11106997408367272No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA30.11106997408367272No Hit
ACTCAGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAG30.11106997408367272No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT30.11106997408367272No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG30.11106997408367272No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30.11106997408367272No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.11106997408367272No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.11106997408367272No Hit
GGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAC30.11106997408367272No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.11106997408367272No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers