FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCACCTCTGGCCGTAACATGGTGGAACCAGAACAGAGTAGGATGCA13.4482758620689653No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC13.4482758620689653No Hit
CCAGTATATTTTATTGGTGTATTTCTTTTGTAAACGATTAATTATCGAGC13.4482758620689653No Hit
CTTCAGTCTCATCAGCAGATTGAGAGCATGATAGAGGCCGAGTCTGCGGT13.4482758620689653No Hit
GGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTC13.4482758620689653No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG13.4482758620689653No Hit
GGGAAAGTCGACGACAAACACAGAGACTGGTGCACCCCAGCTCTGAAGTC13.4482758620689653No Hit
GCCGGATGGGCCTCTTTGCCATCAGCGGGCACCGTTCCTGCTGATGGAGG13.4482758620689653No Hit
GGCCGGGACAGGGGGGACCCGTATCGGTAACAAACACTGGTGATCGAAGA13.4482758620689653No Hit
AAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAGTGCCCACTTCAATT13.4482758620689653No Hit
CCTGAGCATGGCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGA13.4482758620689653No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC13.4482758620689653No Hit
GAACAGGAACAGGATACACAATGGACACAGTAAACAGAACACACAAATAC13.4482758620689653No Hit
CGCCACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCGGTCTGCT13.4482758620689653No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACAAT13.4482758620689653No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG13.4482758620689653No Hit
GTAGCAAAAAGGTACTTTTTTGGACAGTATGTATATCAAATATTAGCATT13.4482758620689653No Hit
GTTTTCAAAGAATTCCTTGGTCATTTCTTTCTCTTTGAAACAAGACTCGG13.4482758620689653No Hit
AATATATTCAATATGGAGAGAATAAAAGAAATGAGATATATAATGTCGCA13.4482758620689653No Hit
CATCTGCAATCCCATTCGGTTCTGGTAGTACGGCAAATTTTCAAGAAGGT13.4482758620689653No Hit
ATGTAGGGGAGAAAGGGAAGCAGCTGGCTGCGGGACAGAGGCAGTGTCTG13.4482758620689653No Hit
CCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG13.4482758620689653No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG13.4482758620689653No Hit
CTTTCATAAGCAACCCTTGTCCTTCGTCCATTTTCACCCCTCCAGAAATT13.4482758620689653No Hit
ACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCCAATTACA13.4482758620689653No Hit
TTGGTGGCCATCTCCTCTCCAGTGAATGAAAAGATGTGAATGTGTGTCTT13.4482758620689653No Hit
TACTAGTGCTGACCAACAAAGTCTCTATAAGAATGCAGATGCATATGTTT13.4482758620689653No Hit
CGTTCATAATTTTTAATGTCTCCACCATCTTGGAAGGACTAAAATTCTCA13.4482758620689653No Hit
GATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAA13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)