FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA32.013422818791946No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG21.342281879194631No Hit
CACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGTC21.342281879194631No Hit
CTCATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCA21.342281879194631No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG21.342281879194631No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC10.6711409395973155No Hit
CATTTGGAAGTTTGTTTCATGTTTTTGGCTTTCCGTTTCATCGCCGAACG10.6711409395973155No Hit
ACATATCAGAGAACAAGAGCGCTTGTTCGCACCGGAATGGATCCCAGAAT10.6711409395973155No Hit
GAACTTTGACCGCTGATGCCAAAGAGGCCCATCAGGCAATCTGAGGGGGT10.6711409395973155No Hit
GAATCAATAATTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCG10.6711409395973155No Hit
TGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACA10.6711409395973155No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.6711409395973155No Hit
TACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGAT10.6711409395973155No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT10.6711409395973155No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG10.6711409395973155No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC10.6711409395973155No Hit
CATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTA10.6711409395973155No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG10.6711409395973155No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA10.6711409395973155No Hit
CTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTCCTGTA10.6711409395973155No Hit
GTCCATAGACTCCGAGAGCCCTGGGTCCTAAGGGCACAGAATATAAACCA10.6711409395973155No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG10.6711409395973155No Hit
CATCAATACTCCAGCACGGAGACAGTGGAAGTGAGTATTGACCGATTTTT10.6711409395973155No Hit
GCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTAC10.6711409395973155No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC10.6711409395973155No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG10.6711409395973155No Hit
AAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGA10.6711409395973155No Hit
GTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAGTCTGGGCT10.6711409395973155No Hit
CCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAAT10.6711409395973155No Hit
ATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTA10.6711409395973155No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT10.6711409395973155No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA10.6711409395973155No Hit
GACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAAT10.6711409395973155No Hit
GTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGAACCATTTC10.6711409395973155No Hit
ACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGA10.6711409395973155No Hit
ATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTA10.6711409395973155No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA10.6711409395973155No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.6711409395973155No Hit
CGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCCGCTTGTCCTTTTGA10.6711409395973155No Hit
GTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGGTATATTATTCCC10.6711409395973155No Hit
CACACATTCACATCTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCG10.6711409395973155No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10.6711409395973155No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA10.6711409395973155No Hit
GTGGGAGACTTTGGTCGGCACGCTTGCGCATAGTTCCTGTAATCTCAAAT10.6711409395973155No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT10.6711409395973155No Hit
TTCCAGAAGTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG10.6711409395973155No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT10.6711409395973155No Hit
GGATAGTTAACCACCCCGTTTGATAGAAGAATACAGTGATTCAGTAGTGT10.6711409395973155No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT10.6711409395973155No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.6711409395973155No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT10.6711409395973155No Hit
GCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCT10.6711409395973155No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA10.6711409395973155No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC10.6711409395973155No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT10.6711409395973155No Hit
TTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATG10.6711409395973155No Hit
GAAATGATGATGTTGACCAAAGTTTGATTATCGCTGCTAGAAACATAGTA10.6711409395973155No Hit
TGATGGGCCTCTTTGCCATCAGCGGTCAACGTTCCTGCTGATGGATGCTC10.6711409395973155No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC10.6711409395973155No Hit
CCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCC10.6711409395973155No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT10.6711409395973155No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG10.6711409395973155No Hit
GATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTC10.6711409395973155No Hit
TGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCA10.6711409395973155No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA10.6711409395973155No Hit
AAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCC10.6711409395973155No Hit
AGCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAA10.6711409395973155No Hit
GTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGC10.6711409395973155No Hit
GTATGCTAGAGTCCCGTTTTCGGTTGATTACCAACACTACGTCCCCGTGC10.6711409395973155No Hit
GACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTTCTTGAAAT10.6711409395973155No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC10.6711409395973155No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA10.6711409395973155No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG10.6711409395973155No Hit
CACAAAAACTGAAAGATGAGCTAATCCTCAACCCTATTGCTGCCTTGCAG10.6711409395973155No Hit
CTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAAT10.6711409395973155No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA10.6711409395973155No Hit
TCACTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAG10.6711409395973155No Hit
AGATAGCTCAGATCATTGATGCTAAATGCTTCCCGAAATCTCTTCTCTTA10.6711409395973155No Hit
ATTCAATGGTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGA10.6711409395973155No Hit
CTTCTAATAGATGGCCCAGCATCACTGAGTCCTGGGATGATGATGGGCAT10.6711409395973155No Hit
GATATAGACTGTTGAATACATATTTTGCCAGTAAGGTACTGCACACTGCC10.6711409395973155No Hit
GCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTA10.6711409395973155No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10.6711409395973155No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC10.6711409395973155No Hit
GGTTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCA10.6711409395973155No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC10.6711409395973155No Hit
CTTTGTAAGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACA10.6711409395973155No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT10.6711409395973155No Hit
CAAACACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTA10.6711409395973155No Hit
CTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATT10.6711409395973155No Hit
CCACCCAGGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTC10.6711409395973155No Hit
ATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAAC10.6711409395973155No Hit
CTCCACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCA10.6711409395973155No Hit
ACACTAATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG10.6711409395973155No Hit
GGGGCGAATGACCCACGTGGGGATGCCCACACAGCCTGTGGGCTAGCGGC10.6711409395973155No Hit
GTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTT10.6711409395973155No Hit
AATGTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTCGATTGGT10.6711409395973155No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA10.6711409395973155No Hit
CCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA10.6711409395973155No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA10.6711409395973155No Hit
ATGTTTGCCCTCTTATAAATTAAATAAGCCACAAGAGCAACTAGATATCA10.6711409395973155No Hit
GAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGACATCCT10.6711409395973155No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA10.6711409395973155No Hit
GACTGGAAGAGACGAACAGACGAATCCCCAAGCAATAAATGAGGCCATGG10.6711409395973155No Hit
CCCATCAGGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAA10.6711409395973155No Hit
GTTTCTCCCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGAAGTGTTGC10.6711409395973155No Hit
CGGTGGGGCCGCCAGCTCCCCCCGGCCTCGGTGGCAGTGCGTCGTTGTCA10.6711409395973155No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT10.6711409395973155No Hit
GCATTGAGGGCAAGCTTTCCCAAATGTCAAAAGAAGTGAATGCCAAAATT10.6711409395973155No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT10.6711409395973155No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA10.6711409395973155No Hit
GTTCCTTGCGTTTCACTGACTTCTTAGGGAGACAATAGTACGTTCCCTCT10.6711409395973155No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC10.6711409395973155No Hit
GTGCCATATCGCTTGTATGCTTTTACAACAAGGCTAACTACTATCCGGAA10.6711409395973155No Hit
CCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACATTTGCTTA10.6711409395973155No Hit
GCGAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTT10.6711409395973155No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAA10.6711409395973155No Hit
CCTCCATACAGCCGTGGAACAGGAACAGGATACACCATGGACACAGTAAA10.6711409395973155No Hit
CTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGCGA10.6711409395973155No Hit
CATTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTC10.6711409395973155No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG10.6711409395973155No Hit
CTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGTGGGATTCTGT10.6711409395973155No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10.6711409395973155No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA10.6711409395973155No Hit
GGGACAGGATGTGAATATGGAAGTTCTTTTCCCGATTGAAGTGGGGGCAA10.6711409395973155No Hit
GTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGAC10.6711409395973155No Hit
ACACAAATGGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGA10.6711409395973155No Hit
ATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCG10.6711409395973155No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT10.6711409395973155No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA10.6711409395973155No Hit
GTCGAAAGGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCA10.6711409395973155No Hit
TTACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTC10.6711409395973155No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG10.6711409395973155No Hit
GATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAATCAAA10.6711409395973155No Hit
CTTTTTTTATCGCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCA10.6711409395973155No Hit
GCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGC10.6711409395973155No Hit
ATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTT10.6711409395973155No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTAATCAAAGTCTAC10.6711409395973155No Hit
GTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCTTCTTGGTC10.6711409395973155No Hit
GCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCT10.6711409395973155No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT10.6711409395973155No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG10.6711409395973155No Hit
AATCAACCCGAGTGGTTCAGAAACATCCTGAGCATGGCACCTATAATGTT10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers