FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGTGTGACGAACGAGTTGAACCAAGAAGCATAAACCAAAACCCAGCG13.7037037037037033No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT13.7037037037037033No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT13.7037037037037033No Hit
TCACTGGGGACAACACTAAGTGGAATGAAAATCAAAATACTCGAATGTTC13.7037037037037033No Hit
GTACTGAGACCAACTGGAACACTTTGTGCAAAAATGCTTAAATCAAAGTC13.7037037037037033No Hit
GAGGAAGGCTCTATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGT13.7037037037037033No Hit
GTCATACTGTTTAAGGTCTCCAACATATTTGCAGTCATCAAAGTCTACTT13.7037037037037033No Hit
ATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAA13.7037037037037033No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCACGAACTCGCGAGATACTC13.7037037037037033No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA13.7037037037037033No Hit
GCTAATGGGTCTGCTGACCCTGCTGCGCTTATGACTATGTTTCTAGCAGC13.7037037037037033No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA13.7037037037037033No Hit
TTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGAGC13.7037037037037033No Hit
CTGCTGAAGAGCCTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA13.7037037037037033No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13.7037037037037033No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC13.7037037037037033No Hit
GCTTGTGACCGTGACTAGCTATGACGAGTGGGTCAGCGTAGGTCCTTTAC13.7037037037037033No Hit
TCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGCTGCTAATGGGTCTG13.7037037037037033No Hit
AGAATAAACATAGCAATTAAAAATGACAAACCCTCTGCATAAAAAAAAAA13.7037037037037033No Hit
AGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGA13.7037037037037033No Hit
TTTTTGGACAGTATGGATAGCAAATAGTACCATTGCCACAACTACTTCAG13.7037037037037033No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA13.7037037037037033No Hit
ATCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCA13.7037037037037033No Hit
AATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG13.7037037037037033No Hit
CCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTACTTGAAATAG13.7037037037037033No Hit
CAAAATGACCATCGTCAACCTCCACAGCACTATGCTGTTCCTGTGGATAT13.7037037037037033No Hit
GGAGCATCCCCAGGAGCTCTATGACAGCGACGCCCACACAGAAGCCTAGG13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)