FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTT210.526315789473683No Hit
GGTATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGA15.263157894736842No Hit
GGATGGACCAATACATGAGGTTAATGAGCCGAGTGGGTCTCCTCAAACAG15.263157894736842No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCAAAAACAGACTGTGTTCT15.263157894736842No Hit
GTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAACATGTACCCTTTC15.263157894736842No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC15.263157894736842No Hit
TAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTA15.263157894736842No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA15.263157894736842No Hit
CATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATT15.263157894736842No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCACCCC15.263157894736842No Hit
AAATAACATTCGAAGCAACTGGAAATCTAGTGGTACCGAGATATGCATTC15.263157894736842No Hit
GGAGTGGACAGAGTCTCCAGGACCTGCAAGTTAGTGGGCATCAACAGGAG15.263157894736842No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT15.263157894736842No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG15.263157894736842No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC15.263157894736842No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC15.263157894736842No Hit
CCGGAATGGATCCCAGAATGTGCTCTCTAATGCAAGGTTCAACACTTCCC15.263157894736842No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)