FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCTCAGTACC15.263157894736842No Hit
CGCATATAGACTGGTGAATACAGATTTTGCCAGTAAGGTCCTGCCCACTT15.263157894736842No Hit
GGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAGCTTGCGCATAGTTCCTG15.263157894736842No Hit
CATATAGACTGTGGAATCCATAGTGTGCCAGTAAGGGCCTGAGCACTGTC15.263157894736842No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT15.263157894736842No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA15.263157894736842No Hit
TGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC15.263157894736842No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCTGGGTGACTC15.263157894736842No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT15.263157894736842No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC15.263157894736842No Hit
AGCCGGTACACTGGATTCGTAAGGCCACTGTTCCAGCAACTGGGGGATGT15.263157894736842No Hit
CCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGCAGTTAAACTA15.263157894736842No Hit
GCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATG15.263157894736842No Hit
GGGTTTAGCGAGGCAGAGCAGGGCGGCCCGAGGGGAGATTATATGAGAGA15.263157894736842No Hit
ACCATGGACAAAGTAAACAGAACACGTACCAGAAGTGCTTCAATCTATTC15.263157894736842No Hit
CTACTGAATACATAATGAAGGGAGTGTACATATATACGGCCTTGCTCAAT15.263157894736842No Hit
GGAGGAAGGCTCTATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTG15.263157894736842No Hit
GCACCCCAGCTCAACCCGAATGATGGCCCACTTCCTGAGGATAATGAACC15.263157894736842No Hit
ATGGGAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)