FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT110.32728354656352276No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG110.32728354656352276No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA90.2677774471883368No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC90.2677774471883368No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG70.20827134781315082No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA70.20827134781315082No Hit
GTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGAC70.20827134781315082No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG70.20827134781315082No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT70.20827134781315082No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA70.20827134781315082No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC70.20827134781315082No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG60.17851829812555786No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA60.17851829812555786No Hit
GTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAATTAGT60.17851829812555786No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT60.17851829812555786No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG60.17851829812555786No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT60.17851829812555786No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT60.17851829812555786No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT60.17851829812555786No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG50.1487652484379649No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG50.1487652484379649No Hit
GCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAA50.1487652484379649No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG50.1487652484379649No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC50.1487652484379649No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT50.1487652484379649No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA50.1487652484379649No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.1487652484379649No Hit
ATCCAGGAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGG50.1487652484379649No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.1487652484379649No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA50.1487652484379649No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA50.1487652484379649No Hit
AGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATT50.1487652484379649No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC50.1487652484379649No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA50.1487652484379649No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG50.1487652484379649No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.1487652484379649No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT50.1487652484379649No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG40.11901219875037192No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.11901219875037192No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC40.11901219875037192No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG40.11901219875037192No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA40.11901219875037192No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT40.11901219875037192No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC40.11901219875037192No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT40.11901219875037192No Hit
ATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGTGA40.11901219875037192No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG40.11901219875037192No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA40.11901219875037192No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC40.11901219875037192No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC40.11901219875037192No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT40.11901219875037192No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT40.11901219875037192No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA40.11901219875037192No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT40.11901219875037192No Hit
GACTGAAATGGTTCAAATTCCATTTTGTTGTATAACATTGTGGGATCTTG40.11901219875037192No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT40.11901219875037192No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC40.11901219875037192No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA40.11901219875037192No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC40.11901219875037192No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT40.11901219875037192No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT40.11901219875037192No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG40.11901219875037192No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG40.11901219875037192No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA40.11901219875037192No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA40.11901219875037192No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC40.11901219875037192No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.11901219875037192No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG40.11901219875037192No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG40.11901219875037192No Hit
GCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTAC40.11901219875037192No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT40.11901219875037192No Hit
ACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAG40.11901219875037192No Hit
GAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAA40.11901219875037192No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT40.11901219875037192No Hit
GGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGAT40.11901219875037192No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40.11901219875037192No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA40.11901219875037192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG100.0067806584145.05
TTATACT100.0067806584145.04
CCTTATA100.0067806584145.02
CCCTTAT100.0067806584145.01
Produced by FastQC (version 0.11.5)