FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG60.26258205689277897No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG60.26258205689277897No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.2188183807439825No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG50.2188183807439825No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.2188183807439825No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.2188183807439825No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT40.175054704595186No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.175054704595186No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA40.175054704595186No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT40.175054704595186No Hit
TTCCAAGAGAGATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTA40.175054704595186No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG40.175054704595186No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT40.175054704595186No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA40.175054704595186No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.175054704595186No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT40.175054704595186No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.175054704595186No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.13129102844638948No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC30.13129102844638948No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT30.13129102844638948No Hit
GGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC30.13129102844638948No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.13129102844638948No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT30.13129102844638948No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.13129102844638948No Hit
GTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCT30.13129102844638948No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA30.13129102844638948No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG30.13129102844638948No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.13129102844638948No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC30.13129102844638948No Hit
CAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCC30.13129102844638948No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.13129102844638948No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.13129102844638948No Hit
CTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGG30.13129102844638948No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA30.13129102844638948No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.13129102844638948No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG30.13129102844638948No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATA30.13129102844638948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCAT100.006630686144.999981
Produced by FastQC (version 0.11.5)