FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATAAGATCCTTTTTTTTTTTTTTCATTGGAAGGCTTGCCCTTTTGT13.8461538461538463No Hit
GGAAAAATACTGTGTTCTTGAAATAGGAGAAATGCTCTTGATGACTGAGA13.8461538461538463No Hit
GAGTAGAGAATGAGTAGGACTGAGCTAAATAAAAGGATGGCAGTGGGGTG13.8461538461538463No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC13.8461538461538463No Hit
CTCCACCCCAGATGTGCCTTCATCTGCATCTTAACTCAATGCACCTGCAT13.8461538461538463No Hit
CTCTACATCTAATAAAGAAAAGTTATTTTAATCTGAACATAGACTACCTG13.8461538461538463No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT13.8461538461538463No Hit
CTCTTAATCTTAGCTAATCGCAAAATGAGATTCTATAGTGGAGACGCCCC13.8461538461538463No Hit
TAACAAAAGAGAAGAAAGCAGAGCTCCAGGATTGTAAAATTGCTCCCTTG13.8461538461538463No Hit
GTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACCGTGTTAC13.8461538461538463No Hit
TCAGAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGA13.8461538461538463No Hit
ACATCATACATAACAAATAACTGCTAGCATACATTACCAGATTTCACAGA13.8461538461538463No Hit
GGATAGGACCTTTAACTTTGAAACACTACAGAAATCCCTGATTTTCCCAT13.8461538461538463No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTACCACTAACTT13.8461538461538463No Hit
ACATCAAACGACGTCAGACTTAAAGAACTCACGGTTTGCTAAATGGACAC13.8461538461538463No Hit
CCATAAGCCCCCGCCCCCGGGCTTCATGGCAGCTCTGGCCATGGATTAAT13.8461538461538463No Hit
GATTCTTACACTGTTGTCTTTACTGTATATAGCCCATCCACTAACAGGGC13.8461538461538463No Hit
CCCGGGGGCGTTCCTCAGCGGCAGGAAACTATCCCCCCCCTCCCGACCCC13.8461538461538463No Hit
CTCCATCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA13.8461538461538463No Hit
ATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTAATGCATTTGATTGCATC13.8461538461538463No Hit
CTGCAATAGTCACAATAGCCAAACAGGTGCTCAAACACACTGTCAGGTGC13.8461538461538463No Hit
GTATATATGTATGTATATACAGATCTCTGAAGATGTCCATGACCGAAAAA13.8461538461538463No Hit
TAATCAAAAAGTACACATCACGAAGGCAAGAGAAGAGCCCCGCAATCAGA13.8461538461538463No Hit
TACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCA13.8461538461538463No Hit
GTGCAGTGCGGCGGACTGATTTTCAAGATCTAGTTGCGTATGTGAGACCC13.8461538461538463No Hit
ATGTAAGACTGATGCCTTAAAGGCTGTGGCGTCTGGCGCCCGGATGGAGG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)