FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC21.5151515151515151No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG21.5151515151515151No Hit
GTTCCAGGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTT10.7575757575757576No Hit
CATCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATAC10.7575757575757576No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAAT10.7575757575757576No Hit
GGCGAAGAGACAATTGAAGAAAAATTTGAGATTACAGGAACTATGCGCAA10.7575757575757576No Hit
GAAAGATGAGCTAATCCTCAACCCTATTGCTGCCTTGCATATGTCTACGG10.7575757575757576No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC10.7575757575757576No Hit
CTAGTGAGTCATGATATCTGCCACTGGGCGAGTCCCCTGATACTCTCTTT10.7575757575757576No Hit
AAACAACACTAGGGTAAATCAGACATATGTAAACATCAGCAACACCAACT10.7575757575757576No Hit
GTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAGCCATCTGTCTGTGAG10.7575757575757576No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTCATTACCAA10.7575757575757576No Hit
GTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTCTTTCCA10.7575757575757576No Hit
CAGTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGC10.7575757575757576No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA10.7575757575757576No Hit
CATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTA10.7575757575757576No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10.7575757575757576No Hit
TGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTT10.7575757575757576No Hit
GTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTT10.7575757575757576No Hit
GCCTCCTCCCTGTAGTAGAGCCCCTAATGGGGGAGTTGGTAGGGAGGGGG10.7575757575757576No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG10.7575757575757576No Hit
CGGCATCATATTCCATGCTTTTGGCTGGCCCATGGGCGGGCATTACCACA10.7575757575757576No Hit
GCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGC10.7575757575757576No Hit
GCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATG10.7575757575757576No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC10.7575757575757576No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG10.7575757575757576No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT10.7575757575757576No Hit
GAACAATAGCAATGGAGTTAATCAGAATGATCAAACGTGGAATCTATGAC10.7575757575757576No Hit
TTTAATGTCAGTGCTCTTATTAGATAGACTCTCTTATTCAGTCTTTGTTT10.7575757575757576No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT10.7575757575757576No Hit
GGTCGTCTTAGTGAGTGACTGCTGCTCAGCTGGTCATGGGGAAAGTTCGA10.7575757575757576No Hit
AGCTAATGGATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCC10.7575757575757576No Hit
GTGCGAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA10.7575757575757576No Hit
GAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAG10.7575757575757576No Hit
TTCTACATCCAAATGTGCACTGAACTCAAACTCAGTGATTATGATGGACG10.7575757575757576No Hit
GCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGC10.7575757575757576No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC10.7575757575757576No Hit
ACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGA10.7575757575757576No Hit
GATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCAGA10.7575757575757576No Hit
GTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTAC10.7575757575757576No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA10.7575757575757576No Hit
GAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAG10.7575757575757576No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG10.7575757575757576No Hit
CATGAAGCCCGGGGGCGGGGGCTTATGGTGGAGTGGAGAGCCAGAGGCTG10.7575757575757576No Hit
GAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAA10.7575757575757576No Hit
GAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACA10.7575757575757576No Hit
GGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGA10.7575757575757576No Hit
GATTTTGCCAGTAAGGTCCTGCACACTTTCACAATAGAGCCTTCCTCCAC10.7575757575757576No Hit
CTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCA10.7575757575757576No Hit
TCCTAATGTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACAT10.7575757575757576No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA10.7575757575757576No Hit
CTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCT10.7575757575757576No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC10.7575757575757576No Hit
TTCTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.7575757575757576No Hit
CAGTGGATTCGTAAGGACACTGTTCCAGCAAATGCGGGATGTGCTTGGGA10.7575757575757576No Hit
GCCCCCTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAG10.7575757575757576No Hit
CTATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGA10.7575757575757576No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT10.7575757575757576No Hit
CCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTG10.7575757575757576No Hit
CTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTG10.7575757575757576No Hit
TCTTTATTCAGTAGGAGCTACTGAGTGGCTGACAGGAAGGAGAGCCCTGG10.7575757575757576No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT10.7575757575757576No Hit
CTGGTGGGGCGGTGCAGGGCGGAGTGGATTGGGTATGGTGTTGGTGGTTA10.7575757575757576No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG10.7575757575757576No Hit
GAGAGAACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCC10.7575757575757576No Hit
AAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTAATGGACAGG10.7575757575757576No Hit
CTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGT10.7575757575757576No Hit
GGGCAACATCTTCTCCTATTTCATCAAGTTCTATCCAGCTTGAATCAGTC10.7575757575757576No Hit
ATCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGC10.7575757575757576No Hit
ACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATC10.7575757575757576No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA10.7575757575757576No Hit
TCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCA10.7575757575757576No Hit
GTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAA10.7575757575757576No Hit
CTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGG10.7575757575757576No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG10.7575757575757576No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGAGAGCTCAGTTC10.7575757575757576No Hit
GAATAAAAGAACTGATAGATATAATTTCGCAGTCACTCACTAGCGATATA10.7575757575757576No Hit
GGGGCTGCTGTCCGGGGCCCTTGCCGGGGTTTGTTGTCCTGTCTGGTTAG10.7575757575757576No Hit
ATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTT10.7575757575757576No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT10.7575757575757576No Hit
TTCCTGGGACTTGTGTGCGAGAGGCAAGTGTGTGGGGATTGAGGAGGGCT10.7575757575757576No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10.7575757575757576No Hit
GAGAAATCCTCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCAACTG10.7575757575757576No Hit
TCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTC10.7575757575757576No Hit
AATTATAACTGTGTGATCTATTATTAGTCAAGAAAATGACCAAAGTGTGT10.7575757575757576No Hit
GAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATCAATGGCC10.7575757575757576No Hit
CTGCATCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCT10.7575757575757576No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG10.7575757575757576No Hit
GGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAA10.7575757575757576No Hit
AATTTGAATGGGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTC10.7575757575757576No Hit
CTATTAGAAGAGGCCTGATTTTCTCAATTTTCGTCTTTGTTGATTCATTG10.7575757575757576No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT10.7575757575757576No Hit
ATATGAAACATTCAACAGTGTCTGCTATCGTTACTAAGTGAGCTAATCTT10.7575757575757576No Hit
CCCCCGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAA10.7575757575757576No Hit
CCCCAGAACTGTGGGAACACCTGTGCAGGGACACACAGTCAGCCATGGTG10.7575757575757576No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT10.7575757575757576No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG10.7575757575757576No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA10.7575757575757576No Hit
CTTTCAAGTTCTTTGCCCATTTTTAAAACTGGGTTGTTACTGTTACTGAT10.7575757575757576No Hit
ATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCAT10.7575757575757576No Hit
ATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGCAA10.7575757575757576No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG10.7575757575757576No Hit
CATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCC10.7575757575757576No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA10.7575757575757576No Hit
AAGGGGTAGAGGGAGTTGACAGGGGAAGGAACAGGCTCATGGGTGTGACA10.7575757575757576No Hit
GTTTGTGCCATCTTCAATTTCTTTCATCAGTGTTTTCTAGTTTTCAGTGT10.7575757575757576No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT10.7575757575757576No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT10.7575757575757576No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA10.7575757575757576No Hit
TCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCATT10.7575757575757576No Hit
CCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTC10.7575757575757576No Hit
CATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCT10.7575757575757576No Hit
GACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGAAAACTTTAGAGCCTA10.7575757575757576No Hit
GGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATTCAAGCTGGA10.7575757575757576No Hit
ATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACT10.7575757575757576No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT10.7575757575757576No Hit
GTGTGTATGGGCTTGCAGTAGCAAGTGGGCATGACTTTGAAAGGGAAGGG10.7575757575757576No Hit
ATTTTGCTCTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGA10.7575757575757576No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC10.7575757575757576No Hit
GCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGAT10.7575757575757576No Hit
ATCCTATTCCATGCTTTTGGCTGGACAATGGGCTGGCATTACCACAGCAT10.7575757575757576No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT10.7575757575757576No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT10.7575757575757576No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC10.7575757575757576No Hit
AAAGAAATACACCAAGACAATATACTGGTGGGATGGGATCCAATAATCCG10.7575757575757576No Hit
CTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAG10.7575757575757576No Hit
GCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGCCCATCAGG10.7575757575757576No Hit
GTATATGTAACACACCAGGGGTAGCTAAGCCTGAATTTCTGCCTGATTGT10.7575757575757576No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGCAAGCTCTTA10.7575757575757576No Hit
CCTATGCTAGCTGGGAGGAAGAGACGCTTTCTACAGGGTCACCAGGCTTG10.7575757575757576No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers