FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG11.5625No Hit
GGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACATTCAAAAG11.5625No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTAAGTTCATTCA11.5625No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGGAATAG11.5625No Hit
GAGGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACAAG11.5625No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT11.5625No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTATATTCAGCCGAAA11.5625No Hit
GCTAGCCCCTGCTTCCAAGCCATGAGGGAAGTGGGATTTATGCCTTTCTC11.5625No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC11.5625No Hit
CATTAGAATAACTCTTGGAAATGTGCCAAAGCACACAGATTGGAGGATAA11.5625No Hit
ATACATAATGAAGGGAGTGTACATAAATAAGGCCTTGCTCAATGCATCCT11.5625No Hit
ACCAGGGCCTCCCCCATGCTAGAAATTCCAACCGGTCTCCTATATGAACG11.5625No Hit
GTGCATTGGGTTCAGTCGCTGGTTTGACCTATTGCCAAAGTTCAGATCTC11.5625No Hit
CATTAGCTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAAT11.5625No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTAACCAAAAGAACT11.5625No Hit
ACATTAAACAGAAATCAACCGGCAGCAACTTCATTCGCCAAAACAATAGA11.5625No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA11.5625No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATACGAATTCTATTGGTCG11.5625No Hit
CCCCGTGGCCTCGATATCGAAACAGAAACTCGTGGTGGTAAACAACTCGT11.5625No Hit
ATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTAT11.5625No Hit
AGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGA11.5625No Hit
GCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTT11.5625No Hit
CCTCTCAGCGACATCGCCGTGCTTGGGGTCATGTCGGGCAGTATTCCGAT11.5625No Hit
GATCAAACCCAATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAA11.5625No Hit
CTGGAGAGGAGATGGCCACCAAAGCGGACTACACCCTTGACGAAGAGAGC11.5625No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.5625No Hit
GATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAG11.5625No Hit
GGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATA11.5625No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG11.5625No Hit
GACCGGAACGGCGACATAGGGAAAACTTATCTAAGGCTACGTTGCTAATG11.5625No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCAGCTTAAAATG11.5625No Hit
AGAAAGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACAAGGCCA11.5625No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG11.5625No Hit
GAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACA11.5625No Hit
GTTCATGCTCAAGCCTACGCAAAAGATAATAGGCCCTCTTTGCGAGCGAT11.5625No Hit
ACACTAAGTGGAATGAAAATCAAAATACTAGAATGTTCATGGCGATGATT11.5625No Hit
AAGTACAGTTCATTACACTAAGGTATATAAAACTTATTTCGAAAAGGTCG11.5625No Hit
GAAGAAAATTGAGAAAATAAGGCCTCATCTAATAGATGGCACAGCATCAC11.5625No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC11.5625No Hit
ACAACAAGGCAACCAAACGACTTACATTTCTTGGAAAGGAAGCAGGTGCA11.5625No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCAGGTTGATTTCTG11.5625No Hit
TAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGA11.5625No Hit
AGCCTATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGAAAGCTTT11.5625No Hit
GAAAATCGAAACTAAAAATTTAGCTGCAATATCCACACAAGTGGAACTTT11.5625No Hit
GATTGGAGGAGTAAGTATGGTGGACATCCTTAGACCCAATGCAACTGAGG11.5625No Hit
GCTGCACAGGAAGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCAT11.5625No Hit
CCAATCAACTAATCAGAAATGAAAACAGTATGGAGAAAGCTAGCACAACC11.5625No Hit
ATACTCACTACCACACTCTCCGTGCTCGAGTAAACATCTACACCCATATA11.5625No Hit
CTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAACCCCTGGCC11.5625No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCGTTGTTTTTTCTACCCTATTGT11.5625No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG11.5625No Hit
CACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCA11.5625No Hit
CAATTACCTCATGGCTTGGAAGCAGTTGCTAGCAGAGCTACAGGACATTG11.5625No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCCACCGGTCTCCTATA11.5625No Hit
TATCGAAAATCTGATTGGAATGATTGGTATATTGCAAGGCATGACTCAAA11.5625No Hit
GTTTGGGGAGCCTTCAGGAAGGTGGCGAGTCCGTCTTGTGTGGAAGTTTG11.5625No Hit
CAACTGCTAAGTTCCTGGAGGTTACCTGCCGGCTTAGGTCGCCACTAGTC11.5625No Hit
AACAAAGACTGAATAAGAGAGGCTATCTAATAAGAGCACTGAAATTAAAT11.5625No Hit
GAGTCCGTATATTGTATAAGTATGGTCCTCCCTCTGATACTACTGGCCCT11.5625No Hit
CTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGAC11.5625No Hit
TCCCAATGGTAAGCAAATGTAGGACCAAAGAAGGAAGACGGCAAACACAC11.5625No Hit
GTCTAGTGTGTGCCACTTGTGAGCAGATTGCTGATTCACAGCATCGGTCT11.5625No Hit
TGATTGGACAATAAACAGAAATCAACCGGCAGCAACTGCATTGGCCAACC11.5625No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG11.5625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAGT50.0145.06
ATTGGAG50.0145.02
TGGAGGA50.0145.04
GATTGGA50.0145.01
ATCAGTC50.0145.0145
GAGTAAG50.0145.09
GGAGGAG50.0145.05
TTGGAGG50.0145.03
AGGAGTA50.0145.07
GGAGTAA50.0145.08
Produced by FastQC (version 0.11.5)