FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTC13.7037037037037033No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACAACTCGGGGTGATGTCTG13.7037037037037033No Hit
GTATGCTAGAGTCCCGTTTTAGTTTTATTAACCACAAAACCGACCCTTGC13.7037037037037033No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG13.7037037037037033No Hit
GGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTC13.7037037037037033No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT13.7037037037037033No Hit
ATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGA13.7037037037037033No Hit
CGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAAT13.7037037037037033No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13.7037037037037033No Hit
AGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTTTTGT13.7037037037037033No Hit
AGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTG13.7037037037037033No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG13.7037037037037033No Hit
GAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATA13.7037037037037033No Hit
GGTCTATACCGACCAGTGAGTACCCGTCCCTTTCAAAGTAATGCCCACTT13.7037037037037033No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACTTCCCCTTTG13.7037037037037033No Hit
ATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCA13.7037037037037033No Hit
TCTCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC13.7037037037037033No Hit
GTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAA13.7037037037037033No Hit
CTAGAAAATCGAACCGGGCTCCAATATGAACGGCTACTGAATAATATGTC13.7037037037037033No Hit
CGATTCCAATACATTCATCAATCATTAGCTACGACCAATACCGATAAGCT13.7037037037037033No Hit
GCCCCATTGTCTGGGCCAGAAATTCCAATTGTTAGCCAATTGATGCCATC13.7037037037037033No Hit
GAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCAC13.7037037037037033No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT13.7037037037037033No Hit
ATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCAT13.7037037037037033No Hit
GGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGT13.7037037037037033No Hit
CTCACTGACCCGAGACTGGAGCCACACAAATGGGAAAAATACTGTGTTCT13.7037037037037033No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)