FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAG14.3478260869565215No Hit
GTCTTCCCTGGAGCCAACTCTATTAAACAGCTTGTCCCTGGGGCAGGTGG14.3478260869565215No Hit
GCAGGGAGAGTGGGAAAGAGAAAAGAAGGTTTACCACTGAGCAGGGAGCC14.3478260869565215No Hit
ACCGTGGGATCATGACCTGAGCTGAAGGCCGAAGCTTAAACGACTGACCC14.3478260869565215No Hit
ATAAAGGTGTGCACCTTGTTTATTTTTTTCGACATCATGCTACTCTACAC14.3478260869565215No Hit
GTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGAGAACT14.3478260869565215No Hit
GGAGAAATCAGCAACAGGACTGTAATGAGAGCTTGAAATAAACCCAAGGG14.3478260869565215No Hit
GGAGACGGCCGAGCGATTCGGGGTAGTGGCCCTCTACAACCTATATGATC14.3478260869565215No Hit
CATCTGCAAAGTGGCTAATGACTACGTCACGGACATGTTAAAATTAAACA14.3478260869565215No Hit
GTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTT14.3478260869565215No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT14.3478260869565215No Hit
TAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTT14.3478260869565215No Hit
CCTTATGTGGACTCGATCCTAGGACCCTGGGATCACAACCAGAGCTGAAG14.3478260869565215No Hit
GAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACCACGCCCCCT14.3478260869565215No Hit
GCAGATCCCCTGCTGAGCACGGCTCCCAATGCAATCCAATCTTAAAACCC14.3478260869565215No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC14.3478260869565215No Hit
GTGTGGGACAGTGAAGAAACCGTCTTTCTTCCTATTCTTACGGAAATCCT14.3478260869565215No Hit
GGGCATGCCCAGTGGCCAGGGCTGGGGCTTTGGCCCCCTCTTCAGCCCCG14.3478260869565215No Hit
CCTGAAAGCCGAGTACCCGCTCTGACTCGATAGTCACTTTGCACGTAAGT14.3478260869565215No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTT14.3478260869565215No Hit
GTATACTTGGTGCCCTTAAAGTAAATTACCTGGGTAGTTGTGGTTATTTG14.3478260869565215No Hit
GTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTAATTACCAACACTACGT14.3478260869565215No Hit
GTCCCAAGCTGTGGGACAAGGAGAGGGCGACAAGCTGGCAGATAATGCTG14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)