FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R2_D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGTCATCAAGATACAG11.1904761904761905No Hit
GAAAAAATTCTGGCTCTACTCTACCACCTCGACTCTACCATCCCACGTAA11.1904761904761905No Hit
GGACTAGTGTCTGCCACTTGTGAACAGATAGCAGATTCACAGCATCGGTC11.1904761904761905No Hit
GGAAAGGAATTTACACAGCACAGATTAGTCAAGCCGGGGTACAGGAGCTC11.1904761904761905No Hit
ACCTAGAGAAACCCAACAAAATAAAATCAGAGAAGAAACACATTCAAATA11.1904761904761905No Hit
AAACTAGGCTTTTCACAATAAGACAAGAAATGGCCAGTAGGAGTCTATGG11.1904761904761905No Hit
CCCATTCGCTTCACGTAGGCCTGCAAATATTCAAGAAGGAAATCTTTCAG11.1904761904761905No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACACGCGAGATA11.1904761904761905No Hit
GGGCCATATCTCCCTATTCCACCATGTTACGGCCAGAGGTGATACCATCA11.1904761904761905No Hit
ATGATGATTATCGGGGAAGACTTTGTACTCCCCTGAATCCCTTTCTCAGA11.1904761904761905No Hit
ACCCTGGCCATGCAGATCTCAGAGCCAAGGAGGCACAGGATGTGATTATG11.1904761904761905No Hit
CCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGAAGTGATGCACTTAAC11.1904761904761905No Hit
ACTTATTCGTCATCAATCATGTGGGACAACAATGGCACTGAGTAAGTGCT11.1904761904761905No Hit
GGCAAAGGCTATCGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGG11.1904761904761905No Hit
AGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGG11.1904761904761905No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG11.1904761904761905No Hit
GCAATATATTGAGAACACAAGAGTCTGAATGAGCATGTGTAAATGGTTCT11.1904761904761905No Hit
GAGAAACCTTGTTTTACGCACCAATACTCATTCTAGCATGAACGCCTCCA11.1904761904761905No Hit
AAGGACAAGGGTTGCTTATGAAAGTATGTGCAATATCCTCAAAGGAAAAT11.1904761904761905No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGAAAAGAACAATAGGGAA11.1904761904761905No Hit
GGGTGTTTTTTATAATAAAATAAGCAGAAACGATAAAGCTCTTATCTCTT11.1904761904761905No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC11.1904761904761905No Hit
ACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAG11.1904761904761905No Hit
CTACTGGCCATTTCATGTATTAAACTTAAAAGACTACTTATGATACAAGC11.1904761904761905No Hit
GCGTTGCTCTGAGCCTTTTATGTAACAACAGTCAGTGTGCAAATCAGTGT11.1904761904761905No Hit
GGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGA11.1904761904761905No Hit
CTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGAAGGGCATGTT11.1904761904761905No Hit
CTCAGGACCGGTCCCGGGAGGTGAGCCTGCTGCGATCTGGGTGCTTCCCA11.1904761904761905No Hit
GTTAGAGAGAGAGAGAGAGACAGCAAAAGCACGGAGCCGAAGAGAGAGAA11.1904761904761905No Hit
GTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATCAATGGCCCTG11.1904761904761905No Hit
CTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAG11.1904761904761905No Hit
GTTTCAAGGCATGAATACTCAAATATTCCTGGGTGGGATTCTTCAAGGAA11.1904761904761905No Hit
TAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCACTTGGAA11.1904761904761905No Hit
GACAAATAATATCAGCAAACCACACACAACCCCATATGTGCATACATAAG11.1904761904761905No Hit
AAACAAGGATGACCTAATTGTTACCTAAAACACTAAAAACAACTGGAAGC11.1904761904761905No Hit
ACAACCACCAATCCACTAAACAGGCAAGAAAACAGAATCCAGCTGGCTAG11.1904761904761905No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTATCTGTCGCT11.1904761904761905No Hit
GTCCAAATAATAATACTTCTCCCCTGTTCTGGTTCACCACCTGAAAATAG11.1904761904761905No Hit
CCTTGAATGAAACCGGCAATGGCCCCAAATAGCCCTCTAGATTGAAAAGA11.1904761904761905No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.1904761904761905No Hit
ATCCCAAGGGCTCCACTTTCATGATCTAATCACAGGCCCCACCACCCAAT11.1904761904761905No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT11.1904761904761905No Hit
ATAGAAACAAGGCATATTTTCATGAAGGACAATTTAAATTCATTATTTTT11.1904761904761905No Hit
ACACAGGACAGCAGACTCCACCCCAGCTGTTCCTACATCTCGATGTTCAC11.1904761904761905No Hit
GGATGGGAACAGTGACCACAGAAGCAGCTATTGATCTAGTGTGTGCCACT11.1904761904761905No Hit
GATTTGTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACG11.1904761904761905No Hit
GATCTTCAAGATCTCACAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA11.1904761904761905No Hit
GGCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT11.1904761904761905No Hit
AATTTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTAAAG11.1904761904761905No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAA11.1904761904761905No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGTCGTTTTTAAACAATTCG11.1904761904761905No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGCTCTAATGTCGCA11.1904761904761905No Hit
GGCTTTTGCTTCCAGACCTCTTAAATGAATTGGAGAAAATGAGCAATATA11.1904761904761905No Hit
TTAAAAAACAAATCGACACAAAATGAAGGCCCTCCAAAATCAATAGGTCG11.1904761904761905No Hit
CCCCTATGGCACCTAAATGTGTATCTGTAGTCTTTGATGAACAAATGAAG11.1904761904761905No Hit
GAAACTCCCAGCATGAGCTACTCTGGATCATGGAGATGATTACTGGAAGG11.1904761904761905No Hit
AATACATTCAATATGGAGAGAATAAAAGAAATGAGAGATCTAATGTCGCA11.1904761904761905No Hit
ATTCAACTTGGGAATCAAAAACAGATTGAAACATCCAATCAAACAGTCAA11.1904761904761905No Hit
GGGCTGGTGGGGCAGCCGCAAAGGGGAGAAGTTTTATTATTTGGCCAGTG11.1904761904761905No Hit
CTTTTTTATTCCCCTCTTTTTCTTAAATGCTGGGAGAAAAAAAAATCTAG11.1904761904761905No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTGCAGCG11.1904761904761905No Hit
TCCTGTGAGAATACGATGGCCACAATTATAGCATCAGCAATTGACTGCTC11.1904761904761905No Hit
CTAAGACCACTGTGGACAATATGGCCATAATCAAAAAGTACCAATCAGGA11.1904761904761905No Hit
AAAATAATCGAGGAAGACTTTGTAAACGACTGAATCCCTTTGTCAGTCAT11.1904761904761905No Hit
AAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCATTCATAAGATTTCA11.1904761904761905No Hit
GTAGAAACAAGGGTGTTTTATATCATTAAATAACCTGAAACGAGAAAGCT11.1904761904761905No Hit
AACTACAACTACAAGAATATAAAGAAAAAAGAGGGTAACCAAAAACACAG11.1904761904761905No Hit
CAGCACGGTATCACAGTCACTAACACTCTCCTGCATACAGGAAGGTTGCT11.1904761904761905No Hit
GGTCCGCACTCTGCACCGTCTGCAGCCTGTGAGCAATCACCAGCACTGTT11.1904761904761905No Hit
ACACCACTCCCATACGCATCAGGTAGCCCAGCAAAATAACAAGAACCTCA11.1904761904761905No Hit
GATTGCAGCAAATCTGGAACATCTGTTCATCCTAAACAAATCCCCTAAGG11.1904761904761905No Hit
CTAAAAAACCATGTTTCAATCGGGAAGTTTAAACTAAATGCAAGATGACA11.1904761904761905No Hit
GACTGCTTCAAGCTGGATAGAACTTGATGAAATAGGAGAAGATGTGGCCC11.1904761904761905No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG11.1904761904761905No Hit
GGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGG11.1904761904761905No Hit
CAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAG11.1904761904761905No Hit
GGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTAGTTTCATTACCAACACT11.1904761904761905No Hit
GGAGAAGATGTTGCCCCGATTGAACATATCGCAAGCATGACGAGGAACAA11.1904761904761905No Hit
GAGAAGTACCCTCTGGGTGCTTAATCAGTGGCAGCGGTCCCTCTCACTTG11.1904761904761905No Hit
GTGCAGGAGGAGGACCAGGTGCAGGAGCAGGTGCAGCAGCAGTGGCATCA11.1904761904761905No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT11.1904761904761905No Hit
ATATAAAAGAATATACATGATTTTATTTTTGGCTCAATCAAGTTGCAGAC11.1904761904761905No Hit
GAAACTAGACCCTTGCCTTCACATATACAAAAATTAACTCCAAATGGATC11.1904761904761905No Hit
AAAAGGAACGTATCATTTGAGAAATGATACTGATGTGGTGAACTTTGTAA11.1904761904761905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTT50.0145.03
TTTTTTA50.0145.06
TGTTTTT50.0145.04
TTTTTAT50.0145.07
GTTTTTT50.0145.05
TTTTATA50.0145.08
TAACCAC50.0145.0145
GGGTGTT50.0145.01
TTTATAA50.0145.09
GGTGTTT50.0145.02
Produced by FastQC (version 0.11.5)