FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R2_D08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACT11.2345679012345678No Hit
ACTGTGGACCAAATGGCCATAATCAAAAAGTACACGTCAGGACGGCAAGA11.2345679012345678No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCAATTGCA11.2345679012345678No Hit
CTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACAACGGCAAAGGC11.2345679012345678No Hit
GGCAACCAGGAGATTGATCCAGATGAAAGAAAGCGGGAGAGACGACCAGT11.2345679012345678No Hit
GTATTTTTCCTCAACTGTCATACTCCTCAGCATTGTCTCCGAAGAAATAA11.2345679012345678No Hit
TTACCTAGAGAAAGCCAACAAAATAAAAACTGAGAAGACACACATTCACA11.2345679012345678No Hit
GATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCCCATGCACCA11.2345679012345678No Hit
TGCCTGAACAATGAGAAGCAATTTTATTGATTCAGCCGAAAACCCCTCAA11.2345679012345678No Hit
GAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCACTCT11.2345679012345678No Hit
GCCCAATAGCAGACAATCGGATACCTAACGTCAGTCCGAAACAGCCGAAG11.2345679012345678No Hit
CCCAGTGACTCTTGTTCTCTGGGTTGGCATGGGGTCTAATAAGGCGGCCC11.2345679012345678No Hit
GGAAACAAAATGGACACAGTAAAAAGAACACACCAATACTCAGAAAAGGG11.2345679012345678No Hit
GATACTAATAGCACTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAA11.2345679012345678No Hit
GCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCATTGTATACAGAAT11.2345679012345678No Hit
GTCCTGTAGCTCTGCTAGCACCTGGTTCCAAGCCATGAGGTAATTGGGAT11.2345679012345678No Hit
GTCAATGAGGAAAATTGCATCCTACTCTGATCTCGTGGGGCAGCAGCAAA11.2345679012345678No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.2345679012345678No Hit
GTGCCAGAACAATGAGAAGCAAATTTCTAGATTCAGCCGAAAACCCCTCA11.2345679012345678No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACAC11.2345679012345678No Hit
TAACTTATTCGTCATCAAAGATGTGGGAGATCAATCGCCCTGAGTCAGTG11.2345679012345678No Hit
ATTTAGATCGAATGGCCTAAAAGCTAATAAGTCAGGAAGGCTAATAGATT11.2345679012345678No Hit
GACCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTGTGCAGGA11.2345679012345678No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAACAG11.2345679012345678No Hit
GCATTAAGCAAAACCCAGGGATCATTAAACAGGCACTCCTCGATTGAATC11.2345679012345678No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.2345679012345678No Hit
GTATGACAGAGATGAGCCACAGCCCAGATCACAAGCAAGCTGGGTCCAAT11.2345679012345678No Hit
AATTCAGCCGAGAAGATCATTTGAGATAAAGCAGCTGTGGGTTCAAACCC11.2345679012345678No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC11.2345679012345678No Hit
AAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGG11.2345679012345678No Hit
CCCTTCATTATCTATACAGTAGCCCTGCAGTGGGACACTTCTCCTGTAAA11.2345679012345678No Hit
TTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATT11.2345679012345678No Hit
AAGATCGAAGGTTCCAGGTTCAAGGTTGTCCCTAAGTGTCTGAACAATGA11.2345679012345678No Hit
ACCCAGGGATCAATAATCAGGCACTCCTCGATAGCTACATATAGCCCCCC11.2345679012345678No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11.2345679012345678No Hit
ACTGAATACATAATGAAGCGAGTGTACATAAATACGGCCTTGCTCAATGC11.2345679012345678No Hit
CCCATAGACTCCTACAGGCCATTTCTTGTCATAAAGAGAAAAGCCAAGAT11.2345679012345678No Hit
TGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGC11.2345679012345678No Hit
CAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCA11.2345679012345678No Hit
TATAGGAACAAGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCAT11.2345679012345678No Hit
CAGTAAACAGAACACACAAATACTCAGAAAAGGGAAAGTGGACGACAAAC11.2345679012345678No Hit
CCACCATCCTTACTACTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGA11.2345679012345678No Hit
GCTCTAAAGTTTTCAAGGCTGGCGAATTTCGGATGGAGAATTTGGTCGGC11.2345679012345678No Hit
GGCCTCCACCATTCTAGAAATTACAAACGGTCTCCTATATGAAATCCTAG11.2345679012345678No Hit
TTGCAGGAAAGAACACAGATCTTGAGGCTCTCATGGAATGGCTAAAGACA11.2345679012345678No Hit
GTCCTGTAGAATCTGTCCACTCCTGATTGTATTCCCTCATGGTTTGGTGA11.2345679012345678No Hit
GTAACTTCCTATATGTGAGAACCAATGGAACATCCAAGATCAAGATGAAA11.2345679012345678No Hit
ATCCAGTCCACCCATGCATCACATTCACCTGATCCCAATGATAAGCAAAT11.2345679012345678No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACATTGGACTTA11.2345679012345678No Hit
GAGGGCAAGCTTTCCCAAAAGTCAAAAGAACACAACGCAAAAAATGACCC11.2345679012345678No Hit
GCTTCATATAGCCCCCCAAGATCGAAGCTTCCAGGAACCAGGTTGTCACT11.2345679012345678No Hit
GGAATGGATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTG11.2345679012345678No Hit
GTCCCGCACTCGCGAGAAACTCAATAAGACCACTGTGGACCATATGTCCA11.2345679012345678No Hit
TTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT11.2345679012345678No Hit
CCCAAAAGCACCACATTCCCATACACAGGATATCCACCATACAGCCATCT11.2345679012345678No Hit
AAGGCACCAATAAAGTACTCAATCAAAAAGCCAAGACTACGCAAACGCTA11.2345679012345678No Hit
GTAGTGGAGTAACAGTGGTAAAGAACAACAAGATAAACAATGACCTTGGC11.2345679012345678No Hit
ACTTTATCTAGGAGCATTTGCGAAAATATTGAACAGTCTGAGCTCCCAGT11.2345679012345678No Hit
GAATGACAATTGCATCTGTACCTACTTAGCGCTACCTTTCTGACATGACC11.2345679012345678No Hit
CCTCTGGCCGTAACATGCTGGAATAGGAATGGCCCAACAACAAGTACAGT11.2345679012345678No Hit
AGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTCTTTGAAAACAAA11.2345679012345678No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT11.2345679012345678No Hit
GTTGAACCAACATGCATTAAGCAAAACCCAGGGATCATAAAACAGGCACT11.2345679012345678No Hit
AAAGAAGCAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAG11.2345679012345678No Hit
AGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAAGAAAAGAAAATCGAA11.2345679012345678No Hit
GTATTTGTAACACAACAGGGGTAGAGAAGCCTAAATTTGTTCCTGATTTG11.2345679012345678No Hit
TCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATCTCCTGTAGCACTGCT11.2345679012345678No Hit
ATTTTAAGCAGACTTCAGGAATGTGAAGATTCCGTATATTGTATAAGATT11.2345679012345678No Hit
ACACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACTTTGT11.2345679012345678No Hit
ATACCCAATTACAGCAGACAAGAGAATAATGGACATACAACAAGGCAACC11.2345679012345678No Hit
CATCAGGACAATGCTTGCTAGCATATCTGCTCGTATTTGTGTTCGAATCT11.2345679012345678No Hit
AGGGTAGATAAACTAACTCAAGGTCGCCAGACATATGAATGGACATTAAA11.2345679012345678No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG11.2345679012345678No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCATG11.2345679012345678No Hit
AAGCATTAAGCAAAACCCACGGATCATTAATCAGGCACACCTCGATTGCT11.2345679012345678No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11.2345679012345678No Hit
AGAAGAAACAAGGTCGTTTTTAACCAATTCGACACTAATTGATGGCCATC11.2345679012345678No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG11.2345679012345678No Hit
AACAAAAAAACAAAGACAATATAATGTTTGCATTGTCTCCAAACATAATA11.2345679012345678No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG11.2345679012345678No Hit
GTTTAAAGTTATTAAGAGGACCTCGCTCTTAGTCCTGTAGAATGATGTGC11.2345679012345678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCAG50.0145.01
TCAGCCG50.0145.04
AATGGAT50.0145.0145
CCGAGAA50.0145.08
CGAGAAG50.0145.09
AGCCGAG50.0145.06
TTCAGCC50.0145.03
GCCGAGA50.0145.07
ATTCAGC50.0145.02
CAGCCGA50.0145.05
Produced by FastQC (version 0.11.5)