FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCG13.571428571428571No Hit
GCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGCCAATGCTTC13.571428571428571No Hit
AGTGAGAAATGATGATGTTGACCAAAGTTTGATTATAGCTGCTAGAAACA13.571428571428571No Hit
GGTCTATCCAGACAAGTGAGTACCCTTCCCTTTCAAAGTAATGAACACTT13.571428571428571No Hit
CAATTAGCCTTATCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTCT13.571428571428571No Hit
TTCATGATACGGTTTAAAATGACCAGCATCAGACACTTACTAAGTTCTTA13.571428571428571No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT13.571428571428571No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT13.571428571428571No Hit
AAACCCAGGGATCATTAATCATGCACGCCTAGATTGATTCATAAATCCCC13.571428571428571No Hit
CAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCT13.571428571428571No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA13.571428571428571No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTATTGATCCGTCCAGAATCGAA13.571428571428571No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCAAACACCATGTCAAGCTATC13.571428571428571No Hit
CTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTGCGGATAAT13.571428571428571No Hit
CATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGA13.571428571428571No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG13.571428571428571No Hit
GATAAGTGGTGACTAGCACTGACTCAGTGCCATTGATCTCCCACATCATT13.571428571428571No Hit
ATCAAAGGTAGGGATATTAGTATCAGATGGAGGACCAAACTTATACAATA13.571428571428571No Hit
AATAAGCCCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTT13.571428571428571No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA13.571428571428571No Hit
AATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACC13.571428571428571No Hit
CCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA13.571428571428571No Hit
GTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGT13.571428571428571No Hit
AACAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.571428571428571No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13.571428571428571No Hit
GAAATTCGATAATGGACATGATTAAAGAGAGTAATGACCAAGGAAAACCC13.571428571428571No Hit
CCTATATGAACTGCTAGGGAAAAATTACTCGAATAGAATGCAGCACTTCT13.571428571428571No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)