FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG21.3605442176870748No Hit
TCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGG21.3605442176870748No Hit
TGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCC21.3605442176870748No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA10.6802721088435374No Hit
GGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCCCTAAC10.6802721088435374No Hit
AAGCAGTACAAGGAAGGTATATACAAACAAGGGAATGTAGCTGTAATTTC10.6802721088435374No Hit
GAGAGGGGGGGAAGCAGGCTACTCGCTGAGCAGGGAGCCTGATGCGGGGC10.6802721088435374No Hit
CCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAAT10.6802721088435374No Hit
AAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTT10.6802721088435374No Hit
CTCCCGGCGCGTGCGGTCGAGCCGTACAGATAGATGGCCGTACGGCAATG10.6802721088435374No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT10.6802721088435374No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC10.6802721088435374No Hit
GCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCAATTGC10.6802721088435374No Hit
TCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGG10.6802721088435374No Hit
GCTTGAAGCACTCCTATTGAATAGATCTGGGCTGAGCTGACAGCAGTCAG10.6802721088435374No Hit
GAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGG10.6802721088435374No Hit
ATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTT10.6802721088435374No Hit
CCTCATGCTTGCGATATGTTCAATCGGGGCACCATCTTCTCCTATTTCAT10.6802721088435374No Hit
GGCTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTT10.6802721088435374No Hit
CTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGTCC10.6802721088435374No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT10.6802721088435374No Hit
AATAACTATTAAAAAAGATAAAAAAAAGACAAAAAAAATAAATTAATTAC10.6802721088435374No Hit
GTGCTTGGGACATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGC10.6802721088435374No Hit
ATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCAT10.6802721088435374No Hit
TCATCAGGCTGACCACTTGGCTGTTTTGGAGTAATTTGAATGGGTCTATC10.6802721088435374No Hit
CTATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGT10.6802721088435374No Hit
GATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGC10.6802721088435374No Hit
GTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATTACATATA10.6802721088435374No Hit
GTTTCACTGGTTGACTTTATGTTACCAGTGGCAATTCAGCTGTTTTTTCT10.6802721088435374No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG10.6802721088435374No Hit
GTAATAGTTCATTCTCCCTTCTTGACCCCTCACTTTGGGTCTTATTGCTA10.6802721088435374No Hit
CACCAAGACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTG10.6802721088435374No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC10.6802721088435374No Hit
ATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCT10.6802721088435374No Hit
CTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCG10.6802721088435374No Hit
CAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCT10.6802721088435374No Hit
CTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTCCTCTTATGAAA10.6802721088435374No Hit
TAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAAC10.6802721088435374No Hit
GTGGGCGTTGAGTGTTTACGGTTGGGGGTCCATCGGATTCTTTTCGCCGG10.6802721088435374No Hit
GTACACATCAGGAAGGCAAGACAAGAACCCCGCACTCATAATGAAGTGGA10.6802721088435374No Hit
GAGTAGAAAGAAGCTTTATGGAGTGGATGAGGTTTATGGTTGGACACTGA10.6802721088435374No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10.6802721088435374No Hit
TCACTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTC10.6802721088435374No Hit
ACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATT10.6802721088435374No Hit
TTAGGGCCCAGTATCTGCTTCTCCGTTCCAGGGTATTGGAGTCCATGGTT10.6802721088435374No Hit
CTGTCATATTGCTATTGGTGGCTCTTTGCGTTCACCGTTAAAAGATCATG10.6802721088435374No Hit
GTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTG10.6802721088435374No Hit
GAGGGGGAGACCGCACCCTGGCCCGGGCTCTGAACTGCGTGCCTGCTGCG10.6802721088435374No Hit
TTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCT10.6802721088435374No Hit
ACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTC10.6802721088435374No Hit
TTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATTGCATCTGTACC10.6802721088435374No Hit
ATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAATCAAAA10.6802721088435374No Hit
GAAATATTCTTTTAATTTGGAGAGTCTTAACTACTTTCCTCATTCACTAC10.6802721088435374No Hit
GTAGCCAAGTGAACAATGGACTTGAATCAAGGTGGTGAGAAGGTGGAGTC10.6802721088435374No Hit
AATTGAACCATTCTTGAGGACGACACCACGCCCCCTCAGATTGCCTGATG10.6802721088435374No Hit
ATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCT10.6802721088435374No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA10.6802721088435374No Hit
CATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCATATGAACAAATGG10.6802721088435374No Hit
TCCCCAGACCGGGCCACGGGGGACATGAATGTGGCCGAGGCTGGAAGATC10.6802721088435374No Hit
TCATTACTCCTGTCAAACGAAGGCACGATCGGGATCCTTGCCATTTAGTC10.6802721088435374No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG10.6802721088435374No Hit
CTTTGCTGCTGGACAGTCAGTGGTTTCCGTGAAATTAGCGGGCAATTCCT10.6802721088435374No Hit
GTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCC10.6802721088435374No Hit
AATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTG10.6802721088435374No Hit
CGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTG10.6802721088435374No Hit
CAACAAGTACAGTTCATTACCCTAAGGTATATAACTGTCTCTTATACACA10.6802721088435374No Hit
AAACTATTCTCTGTCGCTTTAAAAAAGTGCAGCAATTCATCAATTAAACA10.6802721088435374No Hit
TTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGAC10.6802721088435374No Hit
TTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACT10.6802721088435374No Hit
AGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGA10.6802721088435374No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT10.6802721088435374No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA10.6802721088435374No Hit
ATGTAAATACACTTGTTTCTTTGACCCTCCTGGGTGAGAGTCTTGGAAGC10.6802721088435374No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT10.6802721088435374No Hit
GTGGTGAACTCCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAA10.6802721088435374No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA10.6802721088435374No Hit
GGACAAAAGAAATACACCAAGACAATATACTGGTGGGATGGGCTCCAATC10.6802721088435374No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC10.6802721088435374No Hit
GGGTTTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGAC10.6802721088435374No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCAGCAGGTGCTGCGGTGAAAGGA10.6802721088435374No Hit
TAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACA10.6802721088435374No Hit
ATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACAT10.6802721088435374No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG10.6802721088435374No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG10.6802721088435374No Hit
CAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATA10.6802721088435374No Hit
GAATATATCCTGCTTTGAGATGACTTCCCATTTTTCTTGAGACCCCCTCT10.6802721088435374No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC10.6802721088435374No Hit
GGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTA10.6802721088435374No Hit
GTCGTAGCTCCCGTGTGCGGTTGGCAGCACCCGACTTGCGGTAGAGGCCT10.6802721088435374No Hit
CTTCCAAGCCATGAGGTAATTGGGATTTATGCCATTCTCATGTGGTTTGA10.6802721088435374No Hit
CCCCAGGTACACTCCTCCGGGCTTGCTCATGGTGGTTTCTCAGGCCCCAC10.6802721088435374No Hit
ATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGAAG10.6802721088435374No Hit
CTGTAGACGAGAGTCAAACTAATCAGAACACTGCAATTCTATACAAAGAG10.6802721088435374No Hit
CTAGAAAGCATTGACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAAT10.6802721088435374No Hit
GATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATG10.6802721088435374No Hit
GTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTT10.6802721088435374No Hit
TGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGT10.6802721088435374No Hit
GAACAATATTGTTAAGATGTCTATACTACCCAAAATCTACATATTTGATG10.6802721088435374No Hit
ACTTACAACCATACATGTTAAAGCACTGGTTTACTGTGAAAAGGACAGTG10.6802721088435374No Hit
CACCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGA10.6802721088435374No Hit
GCATGTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGAAC10.6802721088435374No Hit
GCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATTTTGTTGTATAA10.6802721088435374No Hit
TGTATATCCAGCTCGCTGCAGAGGTCCATGCCCGCGCAAAAGATCAAATG10.6802721088435374No Hit
CGCCAAAATTGAACCATTCTTGAGGACGACACCACGCCCCCTCAGATTGC10.6802721088435374No Hit
GGAGTAACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAA10.6802721088435374No Hit
TTCTGGTGCCATATTTTCACCGAGTGCCCACTTCAATTGGCTTGTTCTCT10.6802721088435374No Hit
ATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAA10.6802721088435374No Hit
TCAGCCTCTGAGCCTAAGGCAGAGGCTTATGAGCCCCCCAGGGGCCCCTA10.6802721088435374No Hit
GTGTAAGGTAGCCAACATCTTTGCAGGCATAACCGGCGACTGTGGCTGGT10.6802721088435374No Hit
GCTAAAGACAAGCCCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT10.6802721088435374No Hit
CCCAAGGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCG10.6802721088435374No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC10.6802721088435374No Hit
GAGCTGAGTCAATATGACTGGGAAGCTGAAAGAGCCCTGTGAAAGCAGCC10.6802721088435374No Hit
TTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTG10.6802721088435374No Hit
CTTCAATCTCAGCGTTTCCTGGGTTTCGACTTTCTCTTACTTGATCCATC10.6802721088435374No Hit
TGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGACA10.6802721088435374No Hit
TTGCCAGCCCTCCTTACCCTCAGTCCCAGGCAATCCAGTCGAGTTTCTTT10.6802721088435374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG10.6802721088435374No Hit
GTCTCCTATTTGATCTGCTAGGGAAAAAATTCTCGTAATGAATGCCGCAC10.6802721088435374No Hit
ACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAA10.6802721088435374No Hit
CTGCCTGTTCCGCCGGCTACTGGGAGCCACCTTGTTTTACGGACCAATTC10.6802721088435374No Hit
CTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTAC10.6802721088435374No Hit
TCTCCAAGGAGTTCACCACGGTGTTTTGATTCTATTTTCTCTTTCATTCC10.6802721088435374No Hit
GCTGGGTTTTCATTTGGTCTCATCAGGCTGACCACTTGGCTGTTTTGGAG10.6802721088435374No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA10.6802721088435374No Hit
ATCTACTATACATCAAGTGGCCTTTTAAAAATTATGTTCATTTAGTAAAA10.6802721088435374No Hit
CAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATC10.6802721088435374No Hit
GCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTGATAGT10.6802721088435374No Hit
GGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAA10.6802721088435374No Hit
GTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGAGAACT10.6802721088435374No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG10.6802721088435374No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT10.6802721088435374No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC10.6802721088435374No Hit
GTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTCAAAAGGA10.6802721088435374No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCTG10.6802721088435374No Hit
TCCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGG10.6802721088435374No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC10.6802721088435374No Hit
CCCAAATACAGACCCACAGCTAGTCACAGCAGGACAGACTTCAGGGACTC10.6802721088435374No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT10.6802721088435374No Hit
CCCGAATGCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACC10.6802721088435374No Hit
TTAGTATACTCTCCACTCAAGTATTTCTTGTATTAAGAAAAGGAAGGCTT10.6802721088435374No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG10.6802721088435374No Hit
GTTCTGAAAAGCCCCCTTACTAGGTGAAGGGCTAGCGAAAAAAATGTCTT10.6802721088435374No Hit
GTAGGAGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACA10.6802721088435374No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers