FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA12.564102564102564No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG12.564102564102564No Hit
TGCCTAGTCTGATTAGCATCCTCCATGGCCTCCGCTGCGTGTTCACTCGA12.564102564102564No Hit
AAAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGC12.564102564102564No Hit
CTCAGTGATTATGAGGGACGACTAATCCAGAATAGCATAACAATAGAGAG12.564102564102564No Hit
GCCCAGACTGTTCAAGCTTTTCGCAAATCCTCCTAGCTAAAGATTCACCA12.564102564102564No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGCTGGCAATGAGATACCC12.564102564102564No Hit
GTCCTACATTTGCTTATCATTGGGATCAGCTGAAAGTCATCCATGGCTGC12.564102564102564No Hit
AGGGCTCACGGGCCAACTCACAACACGGCAACTAAGAGGACATGAAGGGT12.564102564102564No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC12.564102564102564No Hit
GGCGGTAACATGGTGGAATAGGAATGGCCCAACTCCAAGTACAGTTCATT12.564102564102564No Hit
ATGTTCAATCGGCGCAACATCTTCTCCTATTTCATCAAGATATATCAAGC12.564102564102564No Hit
TTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACGGAGGGAAGTCC12.564102564102564No Hit
CCATGAGGGAAAACAAGCAGGATTGGACAGATTCTACAGGCCCTGCAGGT12.564102564102564No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGACCAAAATGAGACATCCTCTC12.564102564102564No Hit
CAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAA12.564102564102564No Hit
CACACATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTAAATG12.564102564102564No Hit
AGGGGAGAAATTCCAACCGGTCGCCGATATGAACGGCTAGGGAAATATTT12.564102564102564No Hit
ATGTAAAAAAACAAAATTAAATATTAAAAATAAAAGGATATTGACCCTAA12.564102564102564No Hit
GTGTATGTTAATTTTTTAAAAGGTATAAATAAATAAATCTTTAAAAAAAG12.564102564102564No Hit
CAGCCAAAGAATGTCTTCATGCATTTGATTTCATCATATAGTGGTATTCC12.564102564102564No Hit
GGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTG12.564102564102564No Hit
GTATTCATCTACTCCCAGTTTGCTGACTCTTTTCCCGCTCAGCGGCAGCT12.564102564102564No Hit
GGACTGCAGCGTGGACGCTTTGTCCAAAATGACCTGAGTGGGAATGGGGG12.564102564102564No Hit
GTCTTGCGCTTTGACAGAAGACTCGGCATCAATAATGAGATCAATCTCCT12.564102564102564No Hit
GCCGTAGGGACAAGAGACTGAAATGTTTCAAATTCCATTTTGTTGTATAA12.564102564102564No Hit
GCGGGGAAAGTTCCTGCTGATGGATGCTCTGAAATTAAGTATTGAAGACC12.564102564102564No Hit
GTCCACATATAATACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC12.564102564102564No Hit
GTGTCCGTACGAATCCACTGTTCCGGCTTCTGGTTGCCTTTGGGACAAGC12.564102564102564No Hit
CCATACAGCCATGGAACAGGAACAGTATACACCATGGACACAGTAAACAG12.564102564102564No Hit
TGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTT12.564102564102564No Hit
CCCCTATCCAGTACGGGGTCCATCGAAAATGACGGTCAATAAAGAAACAT12.564102564102564No Hit
AACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGC12.564102564102564No Hit
AATTACCTCATGGCTTGGAAGCAGGTGCTATCAGATCTACAGTAAATTTA12.564102564102564No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC12.564102564102564No Hit
GCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTACACTCCAGGAGGAG12.564102564102564No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGATTTGCCACACATACTGCAT12.564102564102564No Hit
ATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTAC12.564102564102564No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12.564102564102564No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)