FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG60.5633802816901409No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.4694835680751174No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.3755868544600939No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC30.28169014084507044No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA30.28169014084507044No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA30.28169014084507044No Hit
GAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAACCCCTCAAGTTGT30.28169014084507044No Hit
GAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAGCCGGCGGAACAGGC30.28169014084507044No Hit
TTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACAC30.28169014084507044No Hit
GCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGT30.28169014084507044No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC30.28169014084507044No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC30.28169014084507044No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT30.28169014084507044No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG30.28169014084507044No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG20.18779342723004694No Hit
CTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAA20.18779342723004694No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG20.18779342723004694No Hit
AGTATGGAGTTCTCACTCACTGACCCGAGACTGGAGCCACACAAATGGGA20.18779342723004694No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC20.18779342723004694No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACAT20.18779342723004694No Hit
GTCCACATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGAC20.18779342723004694No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG20.18779342723004694No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT20.18779342723004694No Hit
TTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGGAA20.18779342723004694No Hit
ACATGGTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAA20.18779342723004694No Hit
ACAGAAGACTCGGCCTCAATCATGCTCTCAATCTGCTGAAGAGACTGAAG20.18779342723004694No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC20.18779342723004694No Hit
ATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATA20.18779342723004694No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC20.18779342723004694No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC20.18779342723004694No Hit
TTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCA20.18779342723004694No Hit
TTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATGAAAAG20.18779342723004694No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC20.18779342723004694No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA20.18779342723004694No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.18779342723004694No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA20.18779342723004694No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA20.18779342723004694No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT20.18779342723004694No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG20.18779342723004694No Hit
CCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACT20.18779342723004694No Hit
ATTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCG20.18779342723004694No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG20.18779342723004694No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT20.18779342723004694No Hit
ATTTTACAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAG20.18779342723004694No Hit
GCCTTGATCATGCAATCCTCCTGTGAGAATACCATGGCCACAATTATTGC20.18779342723004694No Hit
AAAATACTGTGTTCTTGAAATAGGAGACATGCTCTTGAGGACTGCGATAG20.18779342723004694No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.18779342723004694No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.18779342723004694No Hit
GATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTCT20.18779342723004694No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG20.18779342723004694No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT20.18779342723004694No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)