FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT14.0No Hit
GTGGGAAATTATCCATTACAAAGGACACTTCAGATGGCTCCTTTTAAATT14.0No Hit
GGCCAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGGA14.0No Hit
CAAATGTACGTTGGGTCTTTGATCACAAACGCATAATAATGGGAGAAAAG14.0No Hit
CTGGGGAATTGCCTCTTACCAGTATCCGCAACACTGATCCGCTCAAAGTC14.0No Hit
CTGTACAAGCAGACAGTCCCGTGAGCCTTCCCAGGCAGGACGATGTTTTC14.0No Hit
GGTGTGAGGAAGGAGTGGCACAAAGATGCATTCAGCAAAACCCAGGGATC14.0No Hit
ATGAAAAGGTGCCCAGAGACTAACGTAAGGAGACAGCCACCAAACTCTTG14.0No Hit
CCGTTGAGCAGAGAGCCCAACATGGGGCTCCATCCCAGGACCCTGGGATC14.0No Hit
CTAGAGGGGTATATGTAGTCTTTGATGAACAATTTAAGCGCCATCTGGGA14.0No Hit
CTTCAATCCAATGATCGTAGAGCTTGATGAAACGTCAATGAAAGAATATG14.0No Hit
ATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGGTCCTACA14.0No Hit
GTTCCTAATTCCTCATGTCCAGTCCTTATACTTCTCTCCAGAAGTCTCAC14.0No Hit
ACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACC14.0No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAAATTTAGCTAGGATCATTTGCG14.0No Hit
CATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTC14.0No Hit
GTCCCATTCGCTCTGTACTTTGCCTGGGTCTTTGCTTAGCTGTCTTTTTC14.0No Hit
GCCTAAAAAGGCCAATGTGTACCTTAACACTGTCTAGAAAGAAGTTCTCA14.0No Hit
TACTTATCTGACTGAGCCACCCAGGTGCACCTGATTTCAGAACTTCTAAA14.0No Hit
CATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTG14.0No Hit
CTCCCACCCTGACCTTGGTACCCAAGGGAGAGGGCCAGGCGCTCCCTCAG14.0No Hit
CATTCATTCATTCATTTGACAAGCATTTTTCGAGCACAGTAGACCAGTTT14.0No Hit
GCTTGCTAGAGATCTGGGCTCTGGCTGCGCACTGTCCTACTGGTTAAGGT14.0No Hit
CATGTACCCTTTCCCTAGTCTTGCAATTTTGTTTGAGAACATTATTGTTG14.0No Hit
GTACAGTATGTTTTAAAAAAACTCCTAGGGCCCAGTGCTTTGGGGAAAAG14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)