FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATGAGTATTGGAGTAACAGTGAGAAAGAACAAAATGATAAACAATGC15.263157894736842No Hit
AACTTATCCTGTATTACAAAGAAGAAATAAGGAAAGTTAGGCGCACAGCA15.263157894736842No Hit
GTTCCTATATGTGAGAACCAATGGCACCTCCAAGATCAAGATGAAATGGG15.263157894736842No Hit
CTGCGCCGGGTATCCGGATTCTAGGGCAAGCACCGGGGTGGGCAGGGCTA15.263157894736842No Hit
CTCCACCCCAGATGTGCCTTCATCTGGCTCTTCAGGCACTGCACCTGCAT15.263157894736842No Hit
GAGTAAGAGAAAACATGACCAAGAAGATGGTCCCGCAAAGAACAATAGGG15.263157894736842No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATATCCTCTCTT15.263157894736842No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15.263157894736842No Hit
CTTCAAAATAAACACTGCCTGTTCCGCCTGCTACTGGGAGAAACCTTGTT15.263157894736842No Hit
CTTTTGGCTGGACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATC15.263157894736842No Hit
TCGCCCCACAAGATCGAAGGTTCCAGGTGCCAGGTTGTCCCTAAGTGCCT15.263157894736842No Hit
CCTGGGAGACAACTGGGTTTAATGCAAAATTATCGTTATAGAAGTTAAGC15.263157894736842No Hit
TACTAATAGATGGCACAGCATCACTGACTCCTGGGATGATGATGGGCATG15.263157894736842No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTATCCCATCTTGACCCCTCACTT15.263157894736842No Hit
GGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGA15.263157894736842No Hit
GTTCTTGAGGCTATGGCTTTCCTTGAAGAATCCCACCAAGGAATATTTGA15.263157894736842No Hit
CTGCTTAAAATGGGAGCTAATGGATGATGATTATCTGGGAAGACTTTGTA15.263157894736842No Hit
GAGTTGGTGCATGGGGTTCAGTAGATGGTTTGCCCTATTGCCAAATTTCA15.263157894736842No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCAGATGGCCA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)