FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA190.684931506849315No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG150.5407354001441961No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG140.5046863734679163No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT120.43258832011535686No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC120.43258832011535686No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT110.3965392934390771No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.3965392934390771No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG100.3604902667627974No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.32444124008651765No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG80.2883922134102379No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG80.2883922134102379No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG80.2883922134102379No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG70.25234318673395817No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG70.25234318673395817No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60.21629416005767843No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG60.21629416005767843No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG60.21629416005767843No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA60.21629416005767843No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA60.21629416005767843No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT60.21629416005767843No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT60.21629416005767843No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.1802451333813987No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG50.1802451333813987No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG50.1802451333813987No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC50.1802451333813987No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT50.1802451333813987No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC50.1802451333813987No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC50.1802451333813987No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC50.1802451333813987No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.1802451333813987No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC50.1802451333813987No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT50.1802451333813987No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.14419610670511895No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT40.14419610670511895No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC40.14419610670511895No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC40.14419610670511895No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTAGATTGCAGCACTT40.14419610670511895No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG40.14419610670511895No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC40.14419610670511895No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA40.14419610670511895No Hit
CATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCT40.14419610670511895No Hit
TTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGT40.14419610670511895No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA40.14419610670511895No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG40.14419610670511895No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT40.14419610670511895No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA40.14419610670511895No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT40.14419610670511895No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA40.14419610670511895No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT40.14419610670511895No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA40.14419610670511895No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40.14419610670511895No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC40.14419610670511895No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC40.14419610670511895No Hit
CTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCTCT40.14419610670511895No Hit
CCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCAC40.14419610670511895No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.14419610670511895No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG40.14419610670511895No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC30.10814708002883922No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG30.10814708002883922No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG30.10814708002883922No Hit
TAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTA30.10814708002883922No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30.10814708002883922No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG30.10814708002883922No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG30.10814708002883922No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA30.10814708002883922No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA30.10814708002883922No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA30.10814708002883922No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT30.10814708002883922No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC30.10814708002883922No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG30.10814708002883922No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC30.10814708002883922No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT30.10814708002883922No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA30.10814708002883922No Hit
GCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAGACTTTGTAAT30.10814708002883922No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC30.10814708002883922No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC30.10814708002883922No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT30.10814708002883922No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTT30.10814708002883922No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30.10814708002883922No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.10814708002883922No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC30.10814708002883922No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCC30.10814708002883922No Hit
GCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGT30.10814708002883922No Hit
CCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTT30.10814708002883922No Hit
GCTCAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGA30.10814708002883922No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC30.10814708002883922No Hit
CCTAGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCT30.10814708002883922No Hit
GATCATTTGAGTTAAAGAAGCTGTGGGATCAAACCCAATCAAAGGTAGGG30.10814708002883922No Hit
GTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGCGGTGAAAGGA30.10814708002883922No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG30.10814708002883922No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT30.10814708002883922No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC30.10814708002883922No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTG30.10814708002883922No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA30.10814708002883922No Hit
GTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAAT30.10814708002883922No Hit
CACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAAC30.10814708002883922No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT30.10814708002883922No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC30.10814708002883922No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC30.10814708002883922No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT30.10814708002883922No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA30.10814708002883922No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT30.10814708002883922No Hit
GTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATTTCCT30.10814708002883922No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA30.10814708002883922No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA30.10814708002883922No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.10814708002883922No Hit
GTCCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCAC30.10814708002883922No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT30.10814708002883922No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG30.10814708002883922No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC30.10814708002883922No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.10814708002883922No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.10814708002883922No Hit
GGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGC30.10814708002883922No Hit
GTGGTGAACAGTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCT30.10814708002883922No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.10814708002883922No Hit
GATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTCCACTCCTGG30.10814708002883922No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.10814708002883922No Hit
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAG30.10814708002883922No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT30.10814708002883922No Hit
AATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTC30.10814708002883922No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA30.10814708002883922No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCT30.10814708002883922No Hit
GTATTCCCTCATGGTTTGGTGCATTCACTATGAGAGCAAAATCGTCGGAT30.10814708002883922No Hit
AAAGAAGTGAACGCCAAAATTGAACCATTCTTGAGGACGACACCACGCCC30.10814708002883922No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA30.10814708002883922No Hit
CTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGAT30.10814708002883922No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC30.10814708002883922No Hit
GACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCGG30.10814708002883922No Hit
GAATGTAGGCTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGG30.10814708002883922No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.10814708002883922No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA100.0063509312147.666661
TAGAAAC100.0067160684144.981813
GTAGAAA100.0067160684144.981812
ACTTTGT100.0067160684144.981818
AACAAGG350.00320171562.1350677