FastQCFastQC Report
Fri 2 Oct 2020
HNCVFBGXG_n01_FD_S3_R2_C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACACCACGCCCCCTCAGATTGCCTGATGGGCCTCTTTGCCATCAGCGG16.25No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA16.25No Hit
AGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGA16.25No Hit
TACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGG16.25No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16.25No Hit
CTGTAGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG16.25No Hit
TTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCT16.25No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC16.25No Hit
ACATTACCTTGATCCCCAAACCAAAGATCCCACAAAAAAGGAGAATTACA16.25No Hit
ATTGGAGTATATGTGAGCACCAATGGAACCTCCAAGATCAAGATGAGGCC16.25No Hit
CACCAAATGTTATTTCTCCATGGGTATGTGCGCTCTGTACATTTAACTTT16.25No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA16.25No Hit
CGTGTGCTGACCGGGGGTGGGAGAAAGGGGGGTGCTGTCTGTTTCCAAGA16.25No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTCCAATCC16.25No Hit
TTTTTAGAGTGAGAACACATACCAGTAGTGGGGAAGGAGCAGAGGGGGGA16.25No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA16.25No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)