FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1680
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC60.35714285714285715No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50.2976190476190476No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG50.2976190476190476No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG40.2380952380952381No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.2380952380952381No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC40.2380952380952381No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA30.17857142857142858No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.17857142857142858No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC30.17857142857142858No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.17857142857142858No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC30.17857142857142858No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.17857142857142858No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30.17857142857142858No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC30.17857142857142858No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA20.11904761904761905No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT20.11904761904761905No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA20.11904761904761905No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20.11904761904761905No Hit
CAGCAGAAGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTG20.11904761904761905No Hit
CTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGA20.11904761904761905No Hit
TATAGGAACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGCGCAT20.11904761904761905No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20.11904761904761905No Hit
CTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA20.11904761904761905No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT20.11904761904761905No Hit
CCATGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAG20.11904761904761905No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT20.11904761904761905No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGACGCA20.11904761904761905No Hit
GGGGAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAA20.11904761904761905No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA20.11904761904761905No Hit
GGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAG20.11904761904761905No Hit
AGGTAGCAGTGTTCGTACTCGGAGATGGGCTTCCCATCCAGCAGCAGCCG20.11904761904761905No Hit
GATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCAC20.11904761904761905No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC20.11904761904761905No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG20.11904761904761905No Hit
CTTTTGTGGTTCCATACAAATTTTAGGATTATTTGTTCTAGTTCTGTGAG20.11904761904761905No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT20.11904761904761905No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG20.11904761904761905No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT20.11904761904761905No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA20.11904761904761905No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.11904761904761905No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC20.11904761904761905No Hit
CTATAAGGAGGCCCTGGAACGGTGCCGGCAGCTGTGGTGGCGACGAGACC20.11904761904761905No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC20.11904761904761905No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA20.11904761904761905No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT20.11904761904761905No Hit
GGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGAGGGAGAGACTCTG20.11904761904761905No Hit
ATCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGG20.11904761904761905No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG20.11904761904761905No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC20.11904761904761905No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT20.11904761904761905No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.11904761904761905No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA20.11904761904761905No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGAC100.006466284145.03
Produced by FastQC (version 0.11.5)