FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15.263157894736842No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGCCTGTGTTCT15.263157894736842No Hit
AATCAAAGGTAGGGCTATTAGTATCAGATGGAGGACCAAACTTATACAAT15.263157894736842No Hit
CTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGC15.263157894736842No Hit
GCTGAGATGTAATCTATTCTAAACTATTCAATATCGCTAACACGCTAATT15.263157894736842No Hit
GCTTGTTGCTAAGTTGGATTCTGTCTAAGCATCTCACCATTCCTTAATGC15.263157894736842No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG15.263157894736842No Hit
GAACAAGGAGGAAATAGAGATAACAACTCACTTTCAAAGAAAAAGGAGAG15.263157894736842No Hit
CCATAGTACCCCCTGCACAATTATCAGATTTTGCTTCCCAGGTTGATCAA15.263157894736842No Hit
CCTGTGGAATTTCCTTGGCCACACTGCCCAATTGAGTAATTGCCCCACGC15.263157894736842No Hit
AACACACCCAATCAAAGGTTTTGATAAAATGTTCAGTAGAAGGACAACAA15.263157894736842No Hit
GGTATAAATAGTAATAATAAGAAAAAAAAAGGCGAAACAACTCAAATACT15.263157894736842No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA15.263157894736842No Hit
CTTATTCTCTTGTCTGCTGTAATTGGGTATCTAATTGACATCATCCACAA15.263157894736842No Hit
ATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGCATGG15.263157894736842No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTATTGTCTTCTTTGC15.263157894736842No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT15.263157894736842No Hit
TGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATGC15.263157894736842No Hit
CATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGA15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)