FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC21.9607843137254901No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA21.9607843137254901No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG21.9607843137254901No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT21.9607843137254901No Hit
CGCGAGATACTCACTAAGACCACTGTGGCCCTTATGTCCATAATAAAAAA10.9803921568627451No Hit
CAGTGCATGTGTGAGGAAGGAGTTGAAACAAGATGCATTAAGCAAAACCC10.9803921568627451No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG10.9803921568627451No Hit
CGGTCAGAGCCACCAAGAAGGACCCACCCTGGCTTCCGGCCCATTCACCA10.9803921568627451No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA10.9803921568627451No Hit
GCTATTAACGTTACGTTCTTATACAAACTGCTATTAATCTGCCAGAAAGA10.9803921568627451No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT10.9803921568627451No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC10.9803921568627451No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC10.9803921568627451No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCATTGTTGATTCCAGCTTTTA10.9803921568627451No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG10.9803921568627451No Hit
GCCCGGGCCCTTGTGCGGAACCCACGAGTCCTACTCCTGGATGAAGCCAC10.9803921568627451No Hit
GGACAAGTGGTGCGGGAGGCTGTTGGAGGGCTGCAGACGGTTCGCAGTTT10.9803921568627451No Hit
GCAGATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAA10.9803921568627451No Hit
AAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGA10.9803921568627451No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA10.9803921568627451No Hit
CCCTGGAGCTAGACTCCTCTATTCACTAGCTGGATGACCTTGGGCAGGTT10.9803921568627451No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA10.9803921568627451No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA10.9803921568627451No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA10.9803921568627451No Hit
GTATAGCATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT10.9803921568627451No Hit
GATTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAG10.9803921568627451No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA10.9803921568627451No Hit
GGGCAATGGTGATGCCCCCAATCTCTGAGTCGCTGAAGCGAAGGAGGAAT10.9803921568627451No Hit
GAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC10.9803921568627451No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC10.9803921568627451No Hit
GGAGAGTGCAGTCCCCGACGGTGTATACCACTGAGGTAGACACGTTCGAT10.9803921568627451No Hit
TAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAAC10.9803921568627451No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA10.9803921568627451No Hit
TTTAAAGGTGAAAGCAGGGAGCTCTCCTGGCTGACCAGAGGAGGCTGGGC10.9803921568627451No Hit
ACTCAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCCCTATGGCACCTA10.9803921568627451No Hit
ATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCA10.9803921568627451No Hit
CTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCT10.9803921568627451No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA10.9803921568627451No Hit
GTGGCATGCCATCCACACCAATTGACTCTTGTGAGCTGGGTTTTCATTTG10.9803921568627451No Hit
AATGCCCTCTAGGAGTCCTCCCGCCGGAAACCCTCCTGATCCTCTTCTCT10.9803921568627451No Hit
AAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCGTAT10.9803921568627451No Hit
CTTCAGTGCATGTGTGAGGAAGGAGTTGAGCCAAGATGCATTAAGCAAAA10.9803921568627451No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG10.9803921568627451No Hit
CAAGCAGGGAGGGAGGAGCCGTGGCAGGCAAGGCCCACTCCGACTCTGTG10.9803921568627451No Hit
CCTGAGCACCAGGACCTGGTCCGCACTCTGCACCGTCTGCCGCCTGTGAG10.9803921568627451No Hit
GTTCTAATCTTCATTCTTATAATATTGAAAATGTAAGCATTACGAAATCT10.9803921568627451No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT10.9803921568627451No Hit
GGGTTAATATTGAAGTGTTGCACTTGACCCAAGGGTAGTGCTGCGCGCAG10.9803921568627451No Hit
GGTTAGCTAATAGTAGCATTGCCACACCTACATCTGTGGTACTGTCACGA10.9803921568627451No Hit
GGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGT10.9803921568627451No Hit
ACTGTGGACCATATGGCCATAATAAAAAAGAACACAACAGGAAGGCAAGA10.9803921568627451No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT10.9803921568627451No Hit
AAAGTAGACTGTGGGACTGGGACTCAGGAAAGAGGGTGTTGGGGGAGAAG10.9803921568627451No Hit
CAAGATGAGTCTACTAACCGAGGTCGAAACGTCCGGGCTTTCTATCATCC10.9803921568627451No Hit
GCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCC10.9803921568627451No Hit
CTTCTCACCAGCACAGCAGCCCTCCTGAGCAAGCGAAGAAGCCGGTGACC10.9803921568627451No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT10.9803921568627451No Hit
ATATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACAT10.9803921568627451No Hit
CAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCA10.9803921568627451No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT10.9803921568627451No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC10.9803921568627451No Hit
GTCCCGCACTCGCGAGATTCTCACTAAGACCACTGTGGACCATATGGCCA10.9803921568627451No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC10.9803921568627451No Hit
CAACAGAGGAATGCTCTTTAAAAAAAAAAAAAAGGCAAAAGGCTTGCTGT10.9803921568627451No Hit
GGGTGTGGTTTTGGCAGTTGTTATACCAAATGCCGTGTTGGCAATAATTC10.9803921568627451No Hit
GGATAAGGGTCAGCAAAATGGGAGTATAGGAATACTCAAGGGCGGAGAGA10.9803921568627451No Hit
GGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTATGGAG10.9803921568627451No Hit
ACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAG10.9803921568627451No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA10.9803921568627451No Hit
CACTATGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA10.9803921568627451No Hit
GCCCGGAGAGAGTGGTAGGGTGGATTGCCCGATTGTTAAGGGTTAGAGAG10.9803921568627451No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA10.9803921568627451No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA10.9803921568627451No Hit
ACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACT10.9803921568627451No Hit
CAGTGCTGCTGCTTGCCTACAAGCCCATGGAGGGGAACTTTGAAGAGATC10.9803921568627451No Hit
CTGCAATAAGCAGACGATTATAGTATCTAGATTAATCCTCAAACCCATCA10.9803921568627451No Hit
GGCCCCTCCCACTCTGCGGGGGCTGGTGGAATTCCAAGACGTCTCTTTTG10.9803921568627451No Hit
CTCAGAGTGGGGTGGCCCAGAGGAGGCCCTGCAGGGGCAGCCGCTGGCAT10.9803921568627451No Hit
GTGCAAAGACCGTTCATTCTGCCACGGTTTTTGAACCTCAAGTTACGATG10.9803921568627451No Hit
GTGAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTT10.9803921568627451No Hit
GCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTT10.9803921568627451No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.9803921568627451No Hit
GTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTC10.9803921568627451No Hit
TGACCTGAGCCAAAGACAGAAGCTTAACGACTGAGTCATGAAGGCACCCC10.9803921568627451No Hit
GTGTGAAAGGAGATGAGGGAATCCAAGGAGGGATCTCCCAGCTCAGAGCA10.9803921568627451No Hit
CCATTGGTTCTCACATATAGGAACATGGGCCTCGACACTTGGCCTATCGC10.9803921568627451No Hit
GGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATG10.9803921568627451No Hit
ACTTTCAGCTGATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGA10.9803921568627451No Hit
AAATATGGCACCAGAAAAAGTAGACTTTGATGACTGCAAAGATGTTGGAG10.9803921568627451No Hit
GCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAG10.9803921568627451No Hit
ACGTCCCCTTGCCCAATTAGCTCATTTGCCTTCTCTCGTTTTGGAAGATT10.9803921568627451No Hit
ATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGG10.9803921568627451No Hit
ACCTGAGGATAATGAACCAAGTGGGTATGCACAAGTAAACAATGCTGTGG10.9803921568627451No Hit
CATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGT10.9803921568627451No Hit
AGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCG10.9803921568627451No Hit
CTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTC10.9803921568627451No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA10.9803921568627451No Hit
CCCCAGAGCCCAATGCTCTTTCTCAGCATCGTCCGCCTCGCTCGCCAATA10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers