FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT27.4074074074074066No Hit
AAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAATGA13.7037037037037033No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCAAGTCCCTAA13.7037037037037033No Hit
GGCCTACACCATGCTAGAAATTCCAACAGGTCTCCTATATGAACTGCTAG13.7037037037037033No Hit
TATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCAC13.7037037037037033No Hit
AACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAG13.7037037037037033No Hit
CTATGAATGTGCCCTTTTTTTGGAAACAGGGTCTTTGCCAGTGTTACAAA13.7037037037037033No Hit
TGTTTCTACTAATAACCCGAGTAGAAACAAGGTCGTTTTTAAACAATTCG13.7037037037037033No Hit
CCAGTATATTGTCGTGGTGTATTTCTTTAGACCAAGATTCAGTATCGATA13.7037037037037033No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA13.7037037037037033No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTGCAAGGGTGGAA13.7037037037037033No Hit
TTTGAATGGATGTCAACCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA13.7037037037037033No Hit
GCTTGACTCAGACAATTCACATGCATTATTGCATTGATATATGAAAAAGA13.7037037037037033No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA13.7037037037037033No Hit
TCGAGGAAATGTCACTGGCCATTTTAATGAACAACCCTATGCAATAGCAT13.7037037037037033No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG13.7037037037037033No Hit
CTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGTTTGGTGCATT13.7037037037037033No Hit
TGTTGCTACTAATAACCAGAGTAGAACCAAGCTAGTTTATAAACAAGACG13.7037037037037033No Hit
CATAAAGGTGCCATAGGGGAGACACACAAATTCACACGACAACATCATTT13.7037037037037033No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAAACCCTTAATTACTCA13.7037037037037033No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACAATAATTGATGGCCATC13.7037037037037033No Hit
GTATGGATAGCAAATAGTAGAATTGCCACAACTACGTCAGTGCATGTGTG13.7037037037037033No Hit
AGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTG13.7037037037037033No Hit
TTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTGGGG13.7037037037037033No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTACT13.7037037037037033No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC13.7037037037037033No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)