FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGAAAGCAGGGGAAAACAAAAGCAACAAAAAGGAAGGCAATACTAGT12.380952380952381No Hit
CAGCTGTACACGCCAGGAGGAGAAGTGAGAAATGATGCTGTTGCACACAG12.380952380952381No Hit
GACCTGAAGAGCACACAGAATGCCATTGACGAGATTACTAACAAAGTAAA12.380952380952381No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT12.380952380952381No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT12.380952380952381No Hit
GGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT12.380952380952381No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12.380952380952381No Hit
AAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAG12.380952380952381No Hit
TCTCATGTGGTTTGACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTC12.380952380952381No Hit
GGGCCTGTAGAATCGGTCCACTCCTGCTTGTATTCCAGCCTGTTTTGGTG12.380952380952381No Hit
GGAAATTGTTCTATAAATAGATGTATTATGCCCCAATACTGAGCATCAGG12.380952380952381No Hit
GAGCGACCCAGCTTTGGAGTGTATGCAGTAAATGAATCAGCTGCCATGAG12.380952380952381No Hit
TTCCAGTTTTGTTCTTTCTCAAAGACTGCTTTGGCTATTTGGGGTCTTTG12.380952380952381No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG12.380952380952381No Hit
GTAGGTGTACTTCTTGAATAAATTTTATTTGTTCGAAACTATTCGCTTGA12.380952380952381No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12.380952380952381No Hit
ATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGCA12.380952380952381No Hit
GGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGC12.380952380952381No Hit
ACAGAGTAGGATGCAATTTTCCTCATTGACTGTTAATGTGAGAGGATCAG12.380952380952381No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT12.380952380952381No Hit
CCACCATCCAGGGAGCAATTTTACAATCCTGGAGCTCTTCGTTCTTCTCT12.380952380952381No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTATCA12.380952380952381No Hit
CCAGTATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAAT12.380952380952381No Hit
TCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCAT12.380952380952381No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAAAC12.380952380952381No Hit
GACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATCAAAGACTACAGA12.380952380952381No Hit
CTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTCTGTTCTGGT12.380952380952381No Hit
GAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCATCATA12.380952380952381No Hit
ACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATC12.380952380952381No Hit
GTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTCCCTCT12.380952380952381No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT12.380952380952381No Hit
TCACAATTGTTTGGAGAAGATCAACAGCTATTCTCAGAAAGGAAAAAATG12.380952380952381No Hit
GGGGCTGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGG12.380952380952381No Hit
GCTCTCGCCACTTTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATT12.380952380952381No Hit
GCCCACTGGGGGACGGCTGCTGCTGGATGGGAAGCCCATCTCCGAGTACG12.380952380952381No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCAAGGGTTTGT12.380952380952381No Hit
AATCAGAATGATCAAACGTGGAATCAATGACCGAAATTTCTGGAGGGGTG12.380952380952381No Hit
AATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTC12.380952380952381No Hit
TATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGAACAG12.380952380952381No Hit
CCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTCG12.380952380952381No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCTGGGCCCTAGACACCATG12.380952380952381No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGG12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)