FastQCFastQC Report
Sat 26 Sep 2020
HNCVFBGXG_n01_FD_S3_R2_B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNCVFBGXG_n01_FD_S3_R2_B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT100.34746351633078526No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC70.2432244614315497No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA60.20847810979847115No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50.17373175816539263No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.13898540653231412No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA40.13898540653231412No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT40.13898540653231412No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40.13898540653231412No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA40.13898540653231412No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA40.13898540653231412No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT40.13898540653231412No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT40.13898540653231412No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.13898540653231412No Hit
TTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAGA30.10423905489923557No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC30.10423905489923557No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG30.10423905489923557No Hit
GATCTGGGCAGTTGGACCTGGAGCAATTTTCTGGTCTTTGGGGGAGAGGG30.10423905489923557No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC30.10423905489923557No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT30.10423905489923557No Hit
GCCCAAAGGGTAGTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAG30.10423905489923557No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.10423905489923557No Hit
GTTATTGGTATTTTGATAGGTATTACATCAAATATGTAGATTTTGGGTAG30.10423905489923557No Hit
GTTATATGCACTATCTCATCTCAGCCTTATAAAAGCCCCATGTGAGAGCT30.10423905489923557No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA30.10423905489923557No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.10423905489923557No Hit
GTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACT30.10423905489923557No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.10423905489923557No Hit
GTTTGGGAACTAGGTGTCAGGAGTGAGGCCCACTGGGGTCAAGGAACCAA30.10423905489923557No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA30.10423905489923557No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)